ArticlePLoS neglected tropical diseases2015
Yellow Fever 17DD Vaccine Virus Infection Causes Detectable Changes in Chicken Embryos.
Article in PLoS neglected tropical diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 7 citations in OpenAlex.
- Detection of Viral Particles in Paraffinized Tissues and Cell Culture by Indirect Immunofluorescence.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Detection of Viral RNA in Paraffinized Tissues by Nested RT-PCR.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Usutu Virus Infection of Embryonated Chicken Eggs and a Chicken Embryo-Derived Primary Cell Line.Viruses · 2020Article
- Generation of Yellow Fever virus vaccine in skeletal muscle cells of chicken embryos.Memorias do Instituto Oswaldo Cruz · 2019Article
- Concepts in Light Microscopy of Viruses.Viruses · 2018Review
- Kinetic Study of Yellow Fever 17DD Viral Infection in Gallus gallus domesticus Embryos.PloS one · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The yellow fever (YF) 17D vaccine is one of the most effective human vaccines ever created. The YF vaccine has been produced since 1937 in embryonated chicken eggs inoculated with the YF 17D virus. Yet, little information is available about the infection mechanism of YF 17DD virus in this biological model. To better understand this mechanism, we infected embryos of Gallus gallus domesticus and analyzed their histopathology after 72 hours of YF infection. Some embryos showed few apoptotic bodies in infected tissues, suggesting mild focal infection processes. Confocal and super-resolution microscopic analysis allowed us to identify as targets of viral infection: skeletal muscle cells, cardiomyocytes, nervous system cells, renal tubular epithelium, lung parenchyma, and fibroblasts associated with connective tissue in the perichondrium and dermis. The virus replication was heaviest in muscle tissues. In all of these specimens, RT-PCR methods confirmed the presence of replicative intermediate and genomic YF RNA. This clearer characterization of cell targets in chicken embryos paves the way for future development of a new YF vaccine based on a new cell culture system.
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