ArticleeLife2015
Structured illumination with particle averaging reveals novel roles for yeast centrosome components during duplication.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 70 citations in OpenAlex.
- Expansion microscopy reveals characteristic ultrastructural features of pathogenic budding yeast species.Journal of cell science · 2024Article
- A stable microtubule bundle formed through an orchestrated multistep process controls quiescence exit.eLife · 2024Article
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- Ultrastructure expansion microscopy reveals the cellular architecture of budding and fission yeast.Journal of cell science · 2022Article
- Quantitative analysis of nuclear pore complex organization inLife science alliance · 2022Article
- Live cell microscopy: From image to insight.Biophysics reviews · 2022Review
- Computerized fluorescence microscopy of microbial cells.World journal of microbiology & biotechnology · 2021Review
- Fluorescent toys 'n' tools lighting the way in fungal research.FEMS microbiology reviews · 2021Article
- Redistribution of centrosomal proteins by centromeres and Polo kinase controls partial nuclear envelope breakdown in fission yeast.Molecular biology of the cell · 2021Article
- The N-terminus of Sfi1 and yeast centrin Cdc31 provide the assembly site for a new spindle pole body.The Journal of cell biology · 2021Article
- Anatomy of the fungal microtubule organizing center, the spindle pole body.Current opinion in structural biology · 2021Review
- Distribution of γ-tubulin ring complex at themicroPublication biology · 2021Article
- Article
- Super-resolution Microscopy-based Bimolecular Fluorescence Complementation to Study Protein Complex Assembly and Co-localization.Bio-protocol · 2020Article
- SWR1-Independent Association of H2A.Z to the LINC Complex Promotes Meiotic Chromosome Motion.Frontiers in cell and developmental biology · 2020Article
- The stoichiometry of the outer kinetochore is modulated by microtubule-proximal regulatory factors.The Journal of cell biology · 2019Article
- Structure and function of Spc42 coiled-coils in yeast centrosome assembly and duplication.Molecular biology of the cell · 2019Article
- Yeast centrosome components form a noncanonical LINC complex at the nuclear envelope insertion site.The Journal of cell biology · 2019Article
- Novel phosphorylation states of the yeast spindle pole body.Biology open · 2018Article
- Key phosphorylation events in Spc29 and Spc42 guide multiple steps of yeast centrosome duplication.Molecular biology of the cell · 2018Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Duplication of the yeast centrosome (called the spindle pole body, SPB) is thought to occur through a series of discrete steps that culminate in insertion of the new SPB into the nuclear envelope (NE). To better understand this process, we developed a novel two-color structured illumination microscopy with single-particle averaging (SPA-SIM) approach to study the localization of all 18 SPB components during duplication using endogenously expressed fluorescent protein derivatives. The increased resolution and quantitative intensity information obtained using this method allowed us to demonstrate that SPB duplication begins by formation of an asymmetric Sfi1 filament at mitotic exit followed by Mps1-dependent assembly of a Spc29- and Spc42-dependent complex at its tip. Our observation that proteins involved in membrane insertion, such as Mps2, Bbp1, and Ndc1, also accumulate at the new SPB early in duplication suggests that SPB assembly and NE insertion are coupled events during SPB formation in wild-type cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.