ArticleProceedings of the National Academy of Sciences of the United States of America2015
Regional centromeres in the yeast Candida lusitaniae lack pericentromeric heterochromatin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 53 citations in OpenAlex.
- A translocation within the Ogataea species complex alters local subtelomeric chromatin while maintaining overall genome organization.G3 (Bethesda, Md.) · 2026Article
- A Translocation within thebioRxiv : the preprint server for biology · 2026Article
- Centromeres in budding yeasts are conserved in chromosomal location but not in structure.PLoS genetics · 2025Article
- Fungi as models of centromere innovation: from DNA sequence to 3-dimensional arrangement.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- Development of a Shuttle Vector That Transforms at High Frequency for the Emerging Human Fungal Pathogen:Journal of fungi (Basel, Switzerland) · 2024Article
- Behind the scenes: Centromere-driven genomic innovations in fungal pathogens.PLoS pathogens · 2024Article
- Telomere-to-Telomere Genome Assembly of Tibetan Medicinal MushroomJournal of fungi (Basel, Switzerland) · 2023Article
- Peering IntoFrontiers in cellular and infection microbiology · 2022Article
- The Curious Case of Nonrepetitive Centromeric DNA Sequences in Candida auris and Related Species.mBio · 2021Article
- Article
- Clade-specific chromosomal rearrangements and loss of subtelomeric adhesins in Candida auris.Genetics · 2021Article
- Functional and Comparative Analysis of Centromeres Reveals Clade-Specific Genome Rearrangements inmBio · 2021Article
- Spatial inter-centromeric interactions facilitated the emergence of evolutionary new centromeres.eLife · 2020Article
- Polymorphic centromere locations in the pathogenic yeastGenome research · 2020Article
- Evolution of Distinct Responses to Low NADGenetics · 2020Article
- Article
- Article
- Early Diverging Fungus Mucor circinelloides Lacks Centromeric Histone CENP-A and Displays a Mosaic of Point and Regional Centromeres.Current biology : CB · 2019Article
- Transcriptional silencing of centromere repeats by heterochromatin safeguards chromosome integrity.Current genetics · 2019Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Point centromeres are specified by a short consensus sequence that seeds kinetochore formation, whereas regional centromeres lack a conserved sequence and instead are epigenetically inherited. Regional centromeres are generally flanked by heterochromatin that ensures high levels of cohesin and promotes faithful chromosome segregation. However, it is not known whether regional centromeres require pericentromeric heterochromatin. In the yeast Candida lusitaniae, we identified a distinct type of regional centromere that lacks pericentromeric heterochromatin. Centromere locations were determined by ChIP-sequencing of two key centromere proteins, Cse4 and Mif2, and are consistent with bioinformatic predictions. The centromeric DNA sequence was unique for each chromosome and spanned 4-4.5 kbp, consistent with regional epigenetically inherited centromeres. However, unlike other regional centromeres, there was no evidence of pericentromeric heterochromatin in C. lusitaniae. In particular, flanking genes were expressed at a similar level to the rest of the genome, and a URA3 reporter inserted adjacent to a centromere was not repressed. In addition, regions flanking the centromeric core were not associated with hypoacetylated histones or a sirtuin deacetylase that generates heterochromatin in other yeast. Interestingly, the centromeric chromatin had a distinct pattern of histone modifications, being enriched for methylated H3K79 and H3R2 but lacking methylation of H3K4, which is found at other regional centromeres. Thus, not all regional centromeres require flanking heterochromatin.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.