ArticleMolecular biology of the cell2015
Studying early stages of fibronectin fibrillogenesis in living cells by atomic force microscopy.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Evaluation of ToF-SIMS imaging for semi-quantitative mapping of BODIPY-labeled fibronectin surface gradients.The Analyst · 2026Article
- Fibronectin matrix assembly at a glance.Journal of cell science · 2025Review
- Modularity-based mathematical modeling of ligand inter-nanocluster connectivity for unraveling reversible stem cell regulation.Nature communications · 2024Article
- A new mechanism of fibronectin fibril assembly revealed by live imaging and super-resolution microscopy.Journal of cell science · 2022Article
- Hypoxia-induced blood-brain barrier dysfunction is prevented by pericyte-conditioned media via attenuated actomyosin contractility and claudin-5 stabilization.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- HIFα independent mechanisms in renal carcinoma cells modulate divergent outcomes in fibronectin assembly mediated by hypoxia and CoClScientific reports · 2020Article
- ILK supports RhoA/ROCK-mediated contractility of human intestinal epithelial crypt cells by inducing the fibrillogenesis of endogenous soluble fibronectin during the spreading process.BMC molecular and cell biology · 2020Article
- Controlling Fibronectin Fibrillogenesis Using Visible Light.Frontiers in molecular biosciences · 2020Article
- Tspan8 is expressed in breast cancer and regulates E-cadherin/catenin signalling and metastasis accompanied by increased circulating extracellular vesicles.The Journal of pathology · 2019Article
- A Selective Extracellular Matrix Proteomics Approach Identifies Fibronectin Proteolysis by A Disintegrin-like and Metalloprotease Domain with Thrombospondin Type 1 Motifs (ADAMTS16) and Its Impact on Spheroid Morphogenesis.Molecular & cellular proteomics : MCP · 2018Article
- Fibroblast-fibronectin patterning and network formation in 3D fibrin matrices.Matrix biology : journal of the International Society for Matrix Biology · 2017Article
- Stretch-dependent changes in molecular conformation in fibronectin nanofibers.Biomaterials science · 2017Article
- Progress in the Correlative Atomic Force Microscopy and Optical Microscopy.Sensors (Basel, Switzerland) · 2017Review
- AMPK negatively regulates tensin-dependent integrin activity.The Journal of cell biology · 2017Article
- 3D mapping of native extracellular matrix reveals cellular responses to the microenvironment.Journal of structural biology: XArticle
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fibronectin (FN) is an extracellular matrix protein that can be assembled by cells into large fibrillar networks, but the dynamics of FN remodeling and the transition through intermediate fibrillar stages are incompletely understood. Here we used a combination of fluorescence microscopy and time-lapse atomic force microscopy (AFM) to visualize initial stages of FN fibrillogenesis in living fibroblasts at high resolution. Initial FN nanofibrils form within <5 min of cell-matrix contact and subsequently extend at a rate of 0.25 μm/min at sites of cell membrane retraction. FN nanofibrils display a complex linear array of globular features spaced at varying distances, indicating the coexistence of different conformational states within the fibril. In some cases, initial fibrils extended in discrete increments of ∼ 800 nm during a series of cyclical membrane retractions, indicating a stepwise fibrillar extension mechanism. In presence of Mn(2+), a known activator of integrin adhesion to FN, fibrillogenesis was accelerated almost threefold to 0.68 μm/min and fibrillar dimensions were increased, underlining the importance of integrin activation for early FN fibrillogenesis. FN fibrillogenesis visualized by time-lapse AFM thus provides new structural and mechanistic insight into initial steps of cell-driven FN fibrillogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.