ArticleInternational journal of molecular sciences2015
Identification and Characterization of Reference Genes for Normalizing Expression Data from Red Swamp Crawfish Procambarus clarkii.
Article in International journal of molecular sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Physiological and Proteomic Insights into Melatonin-Mediated Regulation of Copper Toxicity in the CrayfishInternational journal of molecular sciences · 2026Article
- Identification and Validation of Tissue-Specific Housekeeping Markers for the Amazon River PrawnGenes · 2025Article
- Reference genes for qPCR expression in black tiger shrimp, Penaeus monodon.Molecular biology reports · 2024Article
- Article
- Selection and validation of reference genes for RT-qPCR-based analyses ofFrontiers in physiology · 2022Article
- Identification of putative ingestion-related olfactory receptor genes in the Chinese mitten crab (Eriocheir japonica sinensis).Genes & genomics · 2021Article
- Comparative transcriptome analysis of the gills of Procambarus clarkii provide novel insights into the response mechanism of ammonia stress tolerance.Molecular biology reports · 2021Article
- Reference gene and tropomyosin expression in mud crab Scylla olivacea, Scylla paramamosain and Scylla tranquebarica.Molecular biology reports · 2020Article
- Validation of Real-time PCR Reference Genes of Muscle Metabolism in Harvested Spiny-Cheek Crayfish (Animals : an open access journal from MDPI · 2020Article
- Lactobacillus acidophilus and L. plantarum improve health status, modulate gut microbiota and innate immune response of marron (Cherax cainii).Scientific reports · 2020Article
- Evaluation of reference genes for quantitative real-time PCR normalization in the scarab beetle Holotrichia oblita.PloS one · 2020Article
- Identification and Evaluation of Reference Genes for Normalization of Gene Expression in Developmental Stages, Sexes, and Tissues of Diaphania caesalis (Lepidoptera, Pyralidae).Journal of insect science (Online) · 2020Article
- RNA sequencing, selection of reference genes and demonstration of feeding RNAi in Thrips tabaci (Lind.) (Thysanoptera: Thripidae).BMC molecular biology · 2019Article
- Marked variations in gut microbiota and some innate immune responses of fresh water crayfish, marron (PeerJ · 2019Article
- Dietary supplementation of black soldier fly (PeerJ · 2019Article
- Validation and Evaluation of Reference Genes for Quantitative Real-Time PCR inInternational journal of molecular sciences · 2018Article
- Suitable reference gene for quantitative real-time PCR analysis of gene expression in gonadal tissues of minnow Puntius sophore under high-temperature stress.BMC genomics · 2017Article
- Evaluation of Housekeeping Genes for Quantitative Real-Time PCR Analysis of Bradysia odoriphaga (Diptera: Sciaridae).International journal of molecular sciences · 2016Article
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7 authors.
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Abstract
qRT-PCR is a widely used technique for rapid and accurate quantification of gene expression data. The use of reference genes for normalization of the expression levels is crucial for accuracy. Several studies have shown that there is no perfect reference gene that is appropriate for use in all experimental conditions, and research on suitable reference genes in red swamp crawfish (Procambarus clarkii) is particularly scarce. In this study, eight commonly used crustacean reference genes were chosen from P. clarkii transcriptome data and investigated as potential candidates for normalization of qRT-PCR data. Expression of these genes under different experimental conditions was examined by qRT-PCR, and the stability of their expression was evaluated using three commonly used statistical algorithms, geNorm, NormFinder and BestKeeper. A final comprehensive ranking determined that EIF and 18S were the optimal reference genes for expression data from different tissues, while TBP and EIF were optimal for expression data from different ovarian developmental stages. To our knowledge, this is the first systematic analysis of reference genes for normalization of qRT-PCR data in P. clarkii. These results will facilitate more accurate and reliable expression studies of this and other crustacean species.
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