ArticleThe Journal of reproduction and development2015
Effect of ganglioside GT1b on the in vitro maturation of porcine oocytes and embryonic development.
Article in The Journal of reproduction and development, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 24 citations in OpenAlex.
- Antioxidant effect of ergothioneine onJournal of veterinary science · 2023Article
- The Association Between Follicular Fluid Sialic Acid Levels, Oocyte Quality, and Pregnancy Rates.Reproductive sciences (Thousand Oaks, Calif.) · 2022Article
- Lipid Signaling During Gamete Maturation.Frontiers in cell and developmental biology · 2022Review
- High-coverage targeted lipidomics revealed dramatic lipid compositional changes in asthenozoospermic spermatozoa and inverse correlation of ganglioside GM3 with sperm motility.Reproductive biology and endocrinology : RB&E · 2021Article
- R-Spondin 2 and WNT/CTNNB1 Signaling Pathways Are Required for Porcine Follicle Development and In Vitro Maturation.Animals : an open access journal from MDPI · 2021Article
- Establishment of 3D Neuro-Organoids Derived from Pig Embryonic Stem-Like Cells.International journal of molecular sciences · 2021Article
- Beneficial Effects of Neurotrophin-4 Supplementation DuringFrontiers in veterinary science · 2021Article
- Effects of Gangliosides on Spermatozoa, Oocytes, and Preimplantation Embryos.International journal of molecular sciences · 2019Review
- Production of transgenic pigs using aJournal of veterinary science · 2018Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ganglioside is an acidic glycosphingolipid with sialic acids residues. This study was performed to investigate the effect and mechanism of ganglioside GT1b in porcine oocytes in the process of in vitro maturation (IVM) and preimplantation development. Metaphase II (MII) rates were significantly (P < 0.05) different between the control group and the 5 nM GT1b treatment group. Intracellular glutathione (GSH) levels in oocytes matured with 5 nM and 20 nM and GT1b decreased significantly (P < 0.05). The 10 nM group showed a significant (P < 0.05) decrease in intracellular reactive oxygen species (ROS) levels compared with the control group. Subsequently, the level of intracellular Ca(2+) in oocytes treated with different concentrations of GT1b was measured. Intracellular Ca(2+) was significantly (P < 0.05) increased with a higher concentration of GT1b in a dose-dependent manner. Real-time PCR was performed and showed that the expression of bradykinin 2 receptor (B2R) and calcium/calmodulin-dependent protein kinase II delta (CaMKIIδ) in cumulus cells was significantly (P < 0.05) decreased in the 20 nM GT1b treatment group. Treatment with 5 nM GT1b significantly (P < 0.05) decreased the expression of CaMKIIδ. In oocytes, treatment with 5 nM GT1b significantly (P < 0.05) decreased CaMKIIγ and POU5F1 (POU domain, class 5, transcription factor 1). However, treatment with 20 nM GT1b significantly (P < 0.05) increased the expression of POU5F1. Finally, embryonic developmental data showed no significant differences in the two experiments (parthenogenesis and in vitro fertilization). In conclusion, the results of the present study indicated that GT1b plays an important role in increasing the nuclear maturation rate and decreasing the intracellular ROS levels during IVM. However, GT1b inhibited maturation of the cytoplasm by maintaining intracellular Ca(2+) in the process of oocyte maturation regardless of the cell cycle stage. Therefore, GT1b is thought to act on another mechanism that controls intracellular Ca(2+).
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