ArticleThe European journal of neuroscience2015
Gad1 mRNA as a reliable indicator of altered GABA release from orexigenic neurons in the hypothalamus.
Article in The European journal of neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 54 citations in OpenAlex.
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- CellSP enables module discovery and visualization for subcellular spatial transcriptomics data.Communications biology · 2025Article
- Multiscale Cell-Cell Interactive Spatial Transcriptomics Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Microglial replacement in a Sandhoff disease mouse model reveals myeloid-derived β-hexosaminidase is necessary for neuronal health.Nature communications · 2025Article
- Low-Glucose Culture Conditions Bias Neuronal Energetics Towards Oxidative Phosphorylation.Journal of neurochemistry · 2025Article
- Fam163a knockdown and mitochondrial stress in the arcuate nucleus of hypothalamus reduce AgRP neuron activity and differentially regulate mitochondrial dynamics in mice.Acta physiologica (Oxford, England) · 2025Article
- Abnormal Local Cortical Functional Connectivity due to Interneuron Dysmaturation after Neonatal Intermittent Hypoxia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Myeloid-derived β-hexosaminidase is essential for neuronal health and lysosome function: implications for Sandhoff disease.bioRxiv : the preprint server for biology · 2024Article
- Investigating the Impact of Fasting and Refeeding on Blood Biochemical Indicators and Transcriptional Profiles in the Hypothalamus and Subcutaneous Adipose Tissue in Geese.Animals : an open access journal from MDPI · 2024Article
- Intracellular spatial transcriptomic analysis toolkit (InSTAnT).Nature communications · 2024Article
- High-Caloric Diets in Adolescence Impair Specific GABAergic Subpopulations, Neurogenesis, and Alter Astrocyte Morphology.International journal of molecular sciences · 2024Article
- GABA tone regulation and its cognitive functions in the brain.Nature reviews. Neuroscience · 2023Review
- Fast neurotransmitter identity of MCH neurons: Do contents depend on context?Frontiers in neuroendocrinology · 2023Review
- Increased intrinsic and synaptic excitability of hypothalamic POMC neurons underlies chronic stress-induced behavioral deficits.Molecular psychiatry · 2023Article
- Seizures regulate the cation-ClFrontiers in neurology · 2023Article
- Age-related Changes of GAD1 mRNA Expression in the Central Inferior Colliculus.Translational medicine of aging · 2023Article
- Satiety Associated with Calorie Restriction and Time-Restricted Feeding: Central Neuroendocrine Integration.Advances in nutrition (Bethesda, Md.) · 2022Review
- Arcuate Nucleus-Dependent Regulation of Metabolism-Pathways to Obesity and Diabetes Mellitus.Endocrine reviews · 2022Review
- Transcriptome analysis reveals the potential roles of long non-coding RNAs in feed efficiency of chicken.Scientific reports · 2022Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The strength of γ-aminobutyric acid (GABA)-mediated inhibitory synaptic input is a principle determinant of neuronal activity. However, because of differences in the number of GABA afferent inputs and the sites of synapses, it is difficult to directly assay for altered GABA transmission between specific cells. The present study tested the hypothesis that the level of mRNA for the GABA synthetic enzyme glutamate decarboxylase (GAD) can provide a reliable proxy for GABA release. This was tested in a mouse hypothalamic circuit important in the regulation of energy balance. Fluorescent in situ hybridization results show that the expression of Gad1 mRNA (encoding the GAD67 enzyme) was increased in hypothalamic neuropeptide Y/agouti-related peptide (NPY/AgRP) neurons after an overnight fast, consistent with the ability of GABA from these neurons to stimulate food intake. Optogenetic studies confirmed that the observed increase in Gad1 mRNA correlated with an increase in the probability of GABA release from NPY/AgRP neurons onto downstream proopiomelanocortin neurons. Likewise, there was an increase in the readily releasable pool of GABA in NPY/AgRP neurons. Selective inhibition of GAD activity in NPY/AgRP neurons decreased GABA release, indicating that GAD67 activity, which is largely dictated by expression level, is a key determinant of GABA release. Altogether, it appears that Gad expression may be a reliable proxy of altered GABAergic transmission. Examining changes in Gad mRNA as a proxy for GABA release may be particularly helpful when the downstream targets are not known or when limited tools exist for detecting GABA release at a particular synapse.
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