ArticleThe Journal of biological chemistry2015
A kissing loop is important for btuB riboswitch ligand sensing and regulatory control.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Complex interplay of riboregulatory mechanisms at theJournal of bacteriology · 2026Article
- Co-transcriptional folding orchestrates sequential multi-effector sensing by a glycine tandem riboswitch.Nature communications · 2026Article
- Twin Small RNAs and Divergent Fates: The Expansive Regulatory Networks of OmrA and OmrB.International journal of molecular sciences · 2025Review
- TheRNA (New York, N.Y.) · 2024Article
- Insights into the cotranscriptional and translational control mechanisms of the Escherichia coli tbpA thiamin pyrophosphate riboswitch.Communications biology · 2024Article
- Riboswitch and small RNAs modulate btuB translation initiation in Escherichia coli and trigger distinct mRNA regulatory mechanisms.Nucleic acids research · 2024Article
- Riboswitches, from cognition to transformation.Synthetic and systems biotechnology · 2023Review
- Structural insights into translation regulation by the THF-II riboswitch.Nucleic acids research · 2023Article
- Article
- Regulation of Gene Expression Through Effector-dependent Conformational Switching by Cobalamin Riboswitches.Journal of molecular biology · 2022Review
- Conformational Ensemble ofThe journal of physical chemistry. B · 2021Article
- The requirement for cobalt in vitamin BBiochimica et biophysica acta. Molecular cell research · 2021Review
- Probing RNA structure and interaction dynamics at the single molecule level.Methods (San Diego, Calif.) · 2019Review
- Triggering nucleic acid nanostructure assembly by conditional kissing interactions.Nucleic acids research · 2018Article
- Transcriptional pausing at the translation start site operates as a critical checkpoint for riboswitch regulation.Nature communications · 2017Article
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Authors and funding
4 authors.
Funding
Abstract
RNA-based genetic regulation is exemplified by metabolite-binding riboswitches that modulate gene expression through conformational changes. Crystal structures show that the Escherichia coli btuB riboswitch contains a kissing loop interaction that is in close proximity to the bound ligand. To analyze the role of the kissing loop interaction in the riboswitch regulatory mechanism, we used RNase H cleavage assays to probe the structure of nascent riboswitch transcripts produced by the E. coli RNA polymerase. By monitoring the folding of the aptamer, kissing loop, and riboswitch expression platform, we established the conformation of each structural component in the absence or presence of bound adenosylcobalamin. We found that the kissing loop interaction is not essential for ligand binding. However, we showed that kissing loop formation improves ligand binding efficiency and is required to couple ligand binding to the riboswitch conformational changes involved in regulating gene expression. These results support a mechanism by which the btuB riboswitch modulates the formation of a tertiary structure to perform metabolite sensing and regulate gene expression.
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