ArticlePlant molecular biology2015
Prediction of Leymus arenarius (L.) antimicrobial peptides based on de novo transcriptome assembly.
Article in Plant molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Defensins of Grasses: A Systematic Review.Biomolecules · 2020Pooled it
- Effects of different additives on fermentation characteristics, nutrient composition and microbial communities of Leymus chinensis silage.BMC microbiology · 2025Article
- A Potent Antibacterial Peptide (P6) from the De Novo Transcriptome of the MicroalgaInternational journal of molecular sciences · 2024Article
- Computational Prediction and Structural Analysis of α-Hairpinins, a Ubiquitous Family of Antimicrobial Peptides, Using the Cysmotif Searcher Pipeline.Antibiotics (Basel, Switzerland) · 2024Article
- Complex of Defense Polypeptides of Wheatgrass (Plants (Basel, Switzerland) · 2024Article
- Identification of a Novel Antimicrobial Peptide From the Ancient Marine Arthropod Chinese Horseshoe Crab,Frontiers in immunology · 2022Article
- Antimicrobial Peptides Derived From Insects Offer a Novel Therapeutic Option to Combat Biofilm: A Review.Frontiers in microbiology · 2021Review
- Predicting Antimicrobial and Other Cysteine-Rich Peptides in 1267 Plant Transcriptomes.Antibiotics (Basel, Switzerland) · 2020Article
- Defense Peptides From the α-Hairpinin Family Are Components of Plant Innate Immunity.Frontiers in plant science · 2020Review
- Non-Specific Lipid Transfer Proteins inPathogens (Basel, Switzerland) · 2019Article
- Article
- Primary Structure Analysis of Antifungal Peptides from Cultivated and Wild Cereals.Plants (Basel, Switzerland) · 2018Review
- Identification and characterization of novel cecropins from the Oxysternon conspicillatum neotropic dung beetle.PloS one · 2017Article
- De novo transcriptome analysis of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) genes in latently infected Se301 cells.Virologica Sinica · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leymus arenarius is a unique wild growing Poaceae plant exhibiting extreme tolerance to environmental conditions. In this study we for the first time performed whole-transcriptome sequencing of lymegrass seedlings using Illumina platform followed by de novo transcriptome assembly and functional annotation. Our goal was to identify transcripts encoding antimicrobial peptides (AMPs), one of the key components of plant innate immunity. Using the custom software developed for this study that predicted AMPs and classified them into families, we revealed more than 160 putative AMPs in lymegrass seedlings. We classified them into 7 families based on their cysteine motifs and sequence similarity. The families included defensins, thionins, hevein-like peptides, snakins, cyclotide, alfa-hairpinins and LTPs. This is the first communication about the presence of almost all known AMP families in trascriptomic data of a single plant species. Additionally, cysteine-rich peptides that potentially represent novel families of AMPs were revealed. We have confirmed by RT-PCR validation the presence of 30 transcripts encoding selected AMPs in lymegrass seedlings. In summary, the presented method of pAMP prediction developed by us can be applied for relatively fast and simple screening of novel components of plant immunity system and is well suited for whole-transcriptome or genome analysis of uncharacterized plants.
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