ArticleThe European journal of neuroscience2016
Optogenetic stimulation of astrocytes in the posterior hypothalamus increases sleep at night in C57BL/6J mice.
Article in The European journal of neuroscience, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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58 citing papers in PubMed, 106 citations in OpenAlex.
- Activity driven sleep dynamics: A conceptual modeling study.PloS one · 2026Article
- Astrocytes at the heart of sleep: from genes to network dynamics.Cellular and molecular life sciences : CMLS · 2025Review
- The Duality of Astrocyte Neuromodulation: Astrocytes Sense Neuromodulators and Are Neuromodulators.Journal of neurochemistry · 2025Review
- Adenosine-Dependent Arousal Induced by Astrocytes in a Brainstem Circuit.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Optogenetic targeting of cortical astrocytes selectively improves NREM sleep in an Alzheimer's disease mouse model.Scientific reports · 2024Article
- Involvement of posterior hypothalamic CaMKII-positive neurons in ADHD-like behaviors in mice.Molecular brain · 2024Article
- Aberrant activation of hippocampal astrocytes causes neuroinflammation and cognitive decline in mice.PLoS biology · 2024Article
- Article
- Unilateral optogenetic stimulation of Lhx6 neurons in the zona incerta increases REM sleep.Sleep · 2024Article
- Hypothalamic MCH Neurons: From Feeding to Cognitive Control.Function (Oxford, England) · 2024Review
- Review
- Local extracellular KProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Astrocytes: new evidence, new models, new roles.Biophysical reviews · 2023Review
- Exploring Astrocyte-Mediated Mechanisms in Sleep Disorders and Comorbidity.Biomedicines · 2023Review
- Activation of astrocytes in the basal forebrain in mice facilitates isoflurane-induced loss of consciousness and prolongs recovery.BMC anesthesiology · 2023Article
- Application of optogenetic glial cells to neuron-glial communication.Frontiers in cellular neuroscience · 2023Review
- Role of astrocytes in sleep deprivation: accomplices, resisters, or bystanders?Frontiers in cellular neuroscience · 2023Review
- Translational approaches to influence sleep and arousal.Brain research bulletin · 2022Review
- Sleep Disturbance and Alzheimer's Disease: The Glial Connection.Neurochemical research · 2022Review
- Crosstalk of Astrocytes and Other Cells during Ischemic Stroke.Life (Basel, Switzerland) · 2022Review
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4 authors at 1 institution in 1 country.
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Abstract
A distributed network of neurons regulates wake, non-rapid eye movement (NREM) sleep, and REM sleep. However, there are also glia in the brain, and there is growing evidence that neurons and astroglia communicate intimately to regulate behaviour. To identify the effect of optogenetic stimulation of astrocytes on sleep, the promoter for the astrocyte-specific cytoskeletal protein, glial fibrillary acidic protein (GFAP) was used to direct the expression of channelrhodopsin-2 (ChR2) and the linked reporter gene, enhanced yellow fluorescent protein (EYFP), in astrocytes. rAAV-GFAP-ChR2 (H134R)-EYFP or rAAV-GFAP-EYFP was microinjected (750 nL) into the posterior hypothalamus (bilateral) of mice. Three weeks later baseline sleep was recorded (0 Hz) and 24 h later optogenetic stimulation applied during the first 6 h of the lights-off period. Mice with ChR2 were given 5, 10 or 30 Hz stimulation for 6 h (10-ms pulses; 1 mW; 1 min on 4 min off). At least 36 h elapsed between the stimulation periods (5, 10, 30 Hz) and although 0 Hz was always first, the order of the other three stimulation rates was randomised. In mice with ChR2 (n = 7), 10 Hz, but not 5 or 30 Hz stimulation increased both NREM and REM sleep during the 6-h period of stimulation. Delta power did not increase. In control mice (no ChR2; n = 5), 10 Hz stimulation had no effect. This study demonstrates that direct stimulation of astrocytes powerfully induces sleep during the active phase of the sleep-wake cycle and underlines the inclusion of astrocytes in network models of sleep-wake regulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.