ArticleScientific reports2015
Real-time and label-free analysis of binding thermodynamics of carbohydrate-protein interactions on unfixed cancer cell surfaces using a QCM biosensor.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 59 citations in OpenAlex.
- Plasmonic glyco-nanoparticles for single-test multiplexed detection and differentiation of cancer cells.Nanoscale · 2026Article
- Label-Free Cell-Based Assay for Characterization of Biomolecules and Receptors.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Flecainide Specifically Targets the Monovalent Countercurrent Through the Cardiac Ryanodine Receptor, While a Dominant Opposing CaInternational journal of molecular sciences · 2024Article
- A comprehensive review of transduction methods of lectin-based biosensors in biomedical applications.Heliyon · 2024Review
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- Recognizing the Less Explored "Active Solid"-"Moving Liquid" Interfaces in Bio/Chemical Sensors.ACS sensors · 2023Review
- Biophysical Approaches for the Characterization of Protein-Metabolite Interactions.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Curvature dependence of BAR protein membrane association and dissociation kinetics.Scientific reports · 2022Article
- Point-of-care human milk testing for maternal secretor status.Analytical and bioanalytical chemistry · 2022Article
- Real time monitoring and evaluation of the inhibition effect of fucoxanthin against α-amylase activity by using QCM-A.Frontiers in nutrition · 2022Article
- Biophysical characterization of melanoma cell phenotype markers during metastatic progression.European biophysics journal : EBJ · 2021Review
- Carbohydrate-Protein Interactions: Advances and Challenges.Communications in information and systems · 2021Article
- Application of QCM in Peptide and Protein-Based Drug Product Development.Molecules (Basel, Switzerland) · 2020Review
- Acoustic Immunosensing of Exosomes Using a Quartz Crystal Microbalance with Dissipation Monitoring.Analytical chemistry · 2020Article
- Kinetic and thermodynamic study of beta-Boswellic acid interaction with Tau protein investigated by surface plasmon resonance and molecular modeling methods.BioImpacts : BI · 2020Article
- Article
- Article
- Mapping molecular binding by means of conformational dynamics measurements.RSC advances · 2018Article
- QCM Biosensor Based on Polydopamine Surface for Real-Time Analysis of the Binding Kinetics of Protein-Protein Interactions.Polymers · 2017Article
- Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel approach to the study of binding thermodynamics and kinetics of carbohydrate-protein interactions on unfixed cancer cell surfaces using a quartz crystal microbalance (QCM) biosensor was developed, in which binding events take place at the cell surface, more closely mimicking a biologically relevant environment. In this study, colon adenocarcinoma cells (KM-12) and ovary adenocarcinoma cells (SKOV-3) grew on the optimized polystyrene-coated biosensor chip without fixation. The association and dissociation between the cell surface carbohydrates and a range of lectins, including WGA, Con A, UEA-I, GS-II, PNA and SBA, were monitored in real time and without label for evaluation of cell surface glycosylation. Furthermore, the thermodynamic and kinetic parameters of the interaction between lectins and cell surface glycan were studied, providing detailed information about the interactions, such as the association rate constant, dissociation rate constant, affinity constant, as well as the changes of entropy, enthalpy and Gibbs free energy. This application provides an insight into the cell surface glycosylation and the complex molecular recognition on the intact cell surface, which may have impacts on disease diagnosis and drug discovery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.