ArticlePLoS neglected tropical diseases2015
Validation of Recombinant Salivary Protein PpSP32 as a Suitable Marker of Human Exposure to Phlebotomus papatasi, the Vector of Leishmania major in Tunisia.
Article in PLoS neglected tropical diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Development of new screening tools to evaluate dog exposure to Phlebotomus tobbi and Phlebotomus papatasi sand flies.Parasites & vectors · 2026Article
- Comparative Analysis ofMicroorganisms · 2024Article
- An overview of the sand fly salivary proteins in vaccine development against leishmaniases.Iranian journal of microbiology · 2022Review
- A Composite Recombinant Salivary Proteins Biomarker for Phlebotomus argentipes Provides a Surveillance Tool Postelimination of Visceral Leishmaniasis in India.The Journal of infectious diseases · 2022Article
- Exposure to Phlebotomus perniciosus sandfly vectors is positively associated with Toscana virus and Leishmania infantum infection in human blood donors in Murcia Region, southeast Spain.Transboundary and emerging diseases · 2022Article
- Some Good and Some Bad: Sand Fly Salivary Proteins in the Control of Leishmaniasis and in Autoimmunity.Frontiers in cellular and infection microbiology · 2022Review
- Vaccination in Leishmaniasis: A Review Article.Iranian biomedical journal · 2022Article
- PpSP32-like protein as a marker of human exposure to Phlebotomus argentipes in Leishmania donovani foci in Bangladesh.International journal for parasitology · 2021Article
- Potential biomarkers of immune protection in human leishmaniasis.Medical microbiology and immunology · 2021Review
- Towards a Sustainable Vector-Control Strategy in the Post Kala-Azar Elimination Era.Frontiers in cellular and infection microbiology · 2021Review
- Implicating bites from a leishmaniasis sand fly vector in the loss of tolerance in pemphigus.JCI insight · 2020Article
- Immunity to vector saliva is compromised by short sand fly seasons in endemic regions with temperate climates.Scientific reports · 2020Article
- Synthetic peptides as a novel approach for detecting antibodies against sand fly saliva.PLoS neglected tropical diseases · 2019Article
- Human antibody reaction against recombinant salivary proteins of Phlebotomus orientalis in Eastern Africa.PLoS neglected tropical diseases · 2018Article
- Seasonal dynamics of canine antibody response to Phlebotomus perniciosus saliva in an endemic area of Leishmania infantum.Parasites & vectors · 2018Article
- Evaluation of the rSP03B sero-strip, a newly proposed rapid test for canine exposure to Phlebotomus perniciosus, vector of Leishmania infantum.PLoS neglected tropical diseases · 2018Article
- Phlebotomus papatasi Yellow-Related and Apyrase Salivary Proteins Are Candidates for Vaccination against Human Cutaneous Leishmaniasis.The Journal of investigative dermatology · 2018Article
- Antibody response to sand fly saliva is a marker of transmission intensity but not disease progression in dogs naturally infected with Leishmania infantum.Parasites & vectors · 2018Article
- Biomarkers for Zoonotic Visceral Leishmaniasis in Latin America.Frontiers in cellular and infection microbiology · 2018Review
- Comparative Evolution of Sand Fly Salivary Protein Families and Implications for Biomarkers of Vector Exposure and Salivary Vaccine Candidates.Frontiers in cellular and infection microbiology · 2018Article
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Authors and funding
14 authors.
Funding
Abstract
backgroundDuring a blood meal, female sand flies, vectors of Leishmania parasites, inject saliva into the host skin. Sand fly saliva is composed of a large variety of components that exert different pharmacological activities facilitating the acquisition of blood by the insect. Importantly, proteins present in saliva are able to elicit the production of specific anti-saliva antibodies, which can be used as markers for exposure to vector bites. Serological tests using total sand fly salivary gland extracts are challenging due to the difficulty of obtaining reproducible salivary gland preparations. Previously, we demonstrated that PpSP32 is the immunodominant salivary antigen in humans exposed to Phlebotomus papatasi bites and established that humans exposed to P. perniciosus bites do not recognize it. METHODOLOGY/PRINCIPAL
findingsHerein, we have validated, in a large cohort of 522 individuals, the use of the Phlebotomus papatasi recombinant salivary protein PpSP32 (rPpSP32) as an alternative method for testing exposure to the bite of this sand fly. We also demonstrated that screening for total anti-rPpSP32 IgG antibodies is sufficient, being comparable in efficacy to the screening for IgG2, IgG4 and IgE antibodies against rPpSP32. Additionally, sera obtained from dogs immunized with saliva of P. perniciosus, a sympatric and widely distributed sand fly in Tunisia, did not recognize rPpSP32 demonstrating its suitability as a marker of exposure to P. papatasi saliva. CONCLUSIONS/SIGNIFICANCE: Our data indicate that rPpSP32 constitutes a useful epidemiological tool to monitor the spatial distribution of P. papatasi in a particular region, to direct control measures against zoonotic cutaneous leishmaniasis, to assess the efficiency of vector control interventions and perhaps to assess the risk of contracting the disease.
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