ArticlePloS one2015
Validation of Reference Genes for RT-qPCR Analysis in Noise-Induced Hearing Loss: A Study in Wistar Rat.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Lack of Oncomodulin Increases ATP-Dependent Calcium Signaling and Susceptibility to Noise in Adult Mice.eNeuro · 2026Article
- Investigation of the Effects of Cadherin 23 and Oncomodulin on Early Progressive Hearing Loss Using a New Oncomodulin Mouse Model.International journal of molecular sciences · 2026Article
- Article
- An electrophysiological early marker of age-related hearing loss in the Wistar rat model.Heliyon · 2024Article
- Oncomodulin regulates spontaneous calcium signalling and maturation of afferent innervation in cochlear outer hair cells.The Journal of physiology · 2023Article
- Selection and validation of reference genes for normalizing qRT‒PCR gene expression studies inFrontiers in plant science · 2023Article
- Kanamycin and Cisplatin Ototoxicity: Differences in Patterns of Oxidative Stress, Antioxidant Enzyme Expression and Hair Cell Loss in the Cochlea.Antioxidants (Basel, Switzerland) · 2022Article
- Downregulation of GJB2 and SLC26A4 genes induced by noise exposure is associated with cochlear damage.Molecular biology reports · 2022Article
- Oncomodulin (OCM) uniquely regulates calcium signaling in neonatal cochlear outer hair cells.Cell calcium · 2022Article
- Low-salt diet increases mRNA expression of aldosterone-regulated transporters in the intermediate portion of the endolymphatic sac.Pflugers Archiv : European journal of physiology · 2022Article
- Age-Related Inflammation and Oxidative Stress in the Cochlea Are Exacerbated by Long-Term, Short-Duration Noise Stimulation.Frontiers in aging neuroscience · 2022Article
- Oral Antioxidant Vitamins and Magnesium Limit Noise-Induced Hearing Loss by Promoting Sensory Hair Cell Survival: Role of Antioxidant Enzymes and Apoptosis Genes.Antioxidants (Basel, Switzerland) · 2020Article
- Validation of reference genes for expression analysis in a murine trauma model combining traumatic brain injury and femoral fracture.Scientific reports · 2020Article
- New Insights on the Effect of TNF Alpha Blockade by Gene Silencing in Noise-Induced Hearing Loss.International journal of molecular sciences · 2020Article
- The rat animal model for noise-induced hearing loss.The Journal of the Acoustical Society of America · 2019Review
- Validation of Reliable Reference Genes for RT-qPCR Studies of Target Gene Expression inFrontiers in microbiology · 2019Article
- Age-Related Hearing Loss Is Accelerated by Repeated Short-Duration Loud Sound Stimulation.Frontiers in neuroscience · 2019Article
- An Oral Combination of Vitamins A, C, E, and MgFrontiers in neuroscience · 2018Article
- The Role of Glia in the Peripheral and Central Auditory System Following Noise Overexposure: Contribution of TNF-α and IL-1β to the Pathogenesis of Hearing Loss.Frontiers in neuroanatomy · 2017Article
- Noise-Induced "Toughening" Effect in Wistar Rats: Enhanced Auditory Brainstem Responses Are Related to Calretinin and Nitric Oxide Synthase Upregulation.Frontiers in neuroanatomy · 2016Article
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5 authors.
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Abstract
The reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) requires adequate normalization in order to ensure accurate results. The use of reference genes is the most common method to normalize RT-qPCR assays; however, many studies have reported that the expression of frequently used reference genes is more variable than expected, depending on experimental conditions. Consequently, proper validation of the stability of reference genes is an essential step when performing new gene expression studies. Despite the fact that RT-qPCR has been widely used to elucidate molecular correlates of noise-induced hearing loss (NIHL), up to date there are no reports demonstrating validation of reference genes for the evaluation of changes in gene expression after NIHL. Therefore, in this study we evaluated the expression of some commonly used reference genes (Arbp, b-Act, b2m, CyA, Gapdh, Hprt1, Tbp, Tfrc and UbC) and examined their suitability as endogenous control genes for RT-qPCR analysis in the adult Wistar rat in response to NIHL. Four groups of rats were noise-exposed to generate permanent cochlear damage. Cochleae were collected at different time points after noise exposure and the expression level of candidate reference genes was evaluated by RT-qPCR using geNorm, NormFinder and BestKeeper software to determine expression stability. The three independent applications revealed Tbp as the most stably expressed reference gene. We also suggest a group of top-ranked reference genes that can be combined to obtain suitable reference gene pairs for the evaluation of the effects of noise on gene expression in the cochlea. These findings provide essential basis for further RT-qPCR analysis in studies of NIHL using Wistar rats as animal model.
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