Evidence map›Paper›PMID 26366722›Full record

ArticleJournal of neuroscience research2015

Sodium-dependent vitamin C transporter-2 mediates vitamin C transport at the cortical nerve terminal.

Marquicia R Pierce, Amita Raj, Katherine M Betke, L Nora Zeidan, Heinrich J G Matthies, James M May

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroscience research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact, top 95% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Marquicia R PierceDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
Amita RajDepartment of Neuroscience, Vanderbilt University, Nashville, Tennessee.
Katherine M BetkeDepartment of Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee.
L Nora ZeidanDepartment of Medicine, Vanderbilt University, Nashville, Tennessee.
Heinrich J G MatthiesDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee.
James M MayDepartment of Medicine, Vanderbilt University, Nashville, Tennessee.
Vanderbilt University · USVanderbilt University Medical Center · US

Funding

Interdisciplinary Training in Rheumatic DiseasesT32AR059039 · NIAMS · VANDERBILT UNIVERSITY · PI Tracy Minan Frech, AMY S MAJOR · 2010 to 2026
$4.3M
Vanderbilt Student Research Training ProgramT35DK007383 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI William Heerman · 1986 to 2026
$4.1M
Vitamin C Transporters in the BrainR01NS057674 · NINDS · VANDERBILT UNIVERSITY · PI MAY, JAMES M. · 2008 to 2011
$1.2M
NIAMS NIH HHS 5T32 AR059039NIAMS NIH HHS T32 AR059039NIDDK NIH HHS T35 DK007383NINDS NIH HHS NS057674NINDS NIH HHS R01 NS057674
6 · The paper itself

Abstract

It has been shown that vitamin C (VC) is transported at synaptic boutons, but how this occurs has not been elucidated. This study investigates the role of the sodium-dependent vitamin C transporter-2 (SVCT2) in transporting VC at the cortical nerve terminal. Immunostaining of cultured mouse superior cervical ganglion cells showed the SVCT2 to be expressed in presynaptic boutons, colocalizing with the vesicular monoamine transporter-2 and the norepinephrine transporter. Immunoblotting of enriched cortical synaptosomes demonstrated that the SVCT2 was enriched in presynaptic fractions, confirming a predominantly presynaptic location. In crude synaptosomes, known inhibitors of SVCT2 inhibited uptake of VC. Furthermore, the kinetic features of VC uptake were consistent with SVCT2-mediated function. VC was also found to efflux from synaptosomes by a mechanism not involving the SVCT2. Indeed, VC efflux was substantially offset by reuptake of VC on the SVCT2. The presence and function of the SVCT2 at the presynaptic nerve terminal suggest that it is the transporter responsible for recovery of VC released into the synaptic cleft.

Indexed as

Analysis of VarianceAnimalsAnimals, NewbornAscorbic AcidBenzofuransCarbon IsotopesCells, CulturedCerebral CortexDisks Large Homolog 4 ProteinGuanylate KinasesImidazolesMembrane ProteinsMiceMice, Inbred C57BLNeuronsNorepinephrine Plasma Membrane Transport ProteinsAscorbic AcidBenzofuransCarbon IsotopesDisks Large Homolog 4 ProteinDlg4 protein, mousefura redGuanylate KinasesImidazolesMembrane ProteinsNorepinephrine Plasma Membrane Transport ProteinsSlc18a2 protein, mouseSlc23a2 protein, mouseSodiumSodium-Coupled Vitamin C TransportersVesicular Monoamine Transport Proteinsantioxidantcortical neuronsgangliamembrane transportsynaptosome

Identifiers

PMID26366722
PMCPMC4618169
OpenAlexW1886224653

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.