ArticlePloS one2015
Antisense Morpholino Oligonucleotides Reduce Neurofilament Synthesis and Inhibit Axon Regeneration in Lamprey Reticulospinal Neurons.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Antisense and Functional Nucleic Acids in Rational Drug Development.Antibiotics (Basel, Switzerland) · 2024Review
- Anti-seed PNAs targeting multiple oncomiRs for brain tumor therapy.Science advances · 2023Article
- Lampreys and spinal cord regeneration: "a very special claim on the interest of zoologists," 1830s-present.Frontiers in cell and developmental biology · 2023Article
- Morpholino studies shed light on the signaling pathways regulating axon regeneration in lampreys.Neural regeneration research · 2022Article
- Next generation miRNA inhibition using short anti-seed PNAs encapsulated in PLGA nanoparticles.Journal of controlled release : official journal of the Controlled Release Society · 2020Article
- Source of Early Regenerating Axons in Lamprey Spinal Cord Revealed by Wholemount Optical Clearing with BABB.Cells · 2020Article
- Mechanisms of Axon Elongation Following CNS Injury: What Is Happening at the Axon Tip?Frontiers in cellular neuroscience · 2020Article
- PTPσ Knockdown in Lampreys Impairs Reticulospinal Axon Regeneration and Neuronal Survival After Spinal Cord Injury.Frontiers in cellular neuroscience · 2020Article
- Serotonin inhibits axonal regeneration of identifiable descending neurons after a complete spinal cord injury in lampreys.Disease models & mechanisms · 2019Article
- RhoA activation in axotomy-induced neuronal death.Experimental neurology · 2018Article
- GABA promotes survival and axonal regeneration in identifiable descending neurons after spinal cord injury in larval lampreys.Cell death & disease · 2018Article
- Highly conserved molecular pathways, including Wnt signaling, promote functional recovery from spinal cord injury in lampreys.Scientific reports · 2018Article
- The role of RhoA in retrograde neuronal death and axon regeneration after spinal cord injury.Neurobiology of disease · 2017Article
- Reducing synuclein accumulation improves neuronal survival after spinal cord injury.Experimental neurology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The sea lamprey has been used as a model for the study of axonal regeneration after spinal cord injury. Previous studies have suggested that, unlike developing axons in mammal, the tips of regenerating axons in lamprey spinal cord are simple in shape, packed with neurofilaments (NFs), and contain very little F-actin. Thus it has been proposed that regeneration of axons in the central nervous system of mature vertebrates is not based on the canonical actin-dependent pulling mechanism of growth cones, but involves an internal protrusive force, perhaps generated by the transport or assembly of NFs in the distal axon. In order to assess this hypothesis, expression of NFs was manipulated by antisense morpholino oligonucleotides (MO). A standard, company-supplied MO was used as control. Axon retraction and regeneration were assessed at 2, 4 and 9 weeks after MOs were applied to a spinal cord transection (TX) site. Antisense MO inhibited NF180 expression compared to control MO. The effect of inhibiting NF expression on axon retraction and regeneration was studied by measuring the distance of axon tips from the TX site at 2 and 4 weeks post-TX, and counting the number of reticulospinal neurons (RNs) retrogradely labeled by fluorescently-tagged dextran injected caudal to the injury at 9 weeks post-TX. There was no statistically significant effect of MO on axon retraction at 2 weeks post-TX. However, at both 4 and 9 weeks post-TX, inhibition of NF expression inhibited axon regeneration.
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