ArticleNature communications2015
Direct observation shows superposition and large scale flexibility within cytoplasmic dynein motors moving along microtubules.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 71 citations in OpenAlex.
- Characterizing dynamics of productive dynein stepping by MINFLUX.Nature structural & molecular biology · 2026Article
- Rational engineering of DNA-nanoparticle motor with high speed and processivity comparable to motor proteins.Nature communications · 2025Article
- Loss ofCells · 2024Article
- Stepping dynamics of dynein characterized by MINFLUX.bioRxiv : the preprint server for biology · 2024Article
- A cryo-ET survey of microtubules and intracellular compartments in mammalian axons.The Journal of cell biology · 2022Article
- HPF1 dynamically controls the PARP1/2 balance between initiating and elongating ADP-ribose modifications.Nature communications · 2021Article
- Three-color single-molecule imaging reveals conformational dynamics of dynein undergoing motility.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- GTP-dependent formation of straight tubulin oligomers leads to microtubule nucleation.The Journal of cell biology · 2021Article
- Characterizing the Core-Shell Architecture of Block Copolymer Nanoparticles with Electron Microscopy: A Multi-Technique Approach.Polymers · 2020Article
- Pac1/LIS1 stabilizes an uninhibited conformation of dynein to coordinate its localization and activity.Nature cell biology · 2020Article
- How Cytoplasmic Dynein Couples ATP Hydrolysis Cycle to Diverse Stepping Motions: Kinetic Modeling.Biophysical journal · 2020Article
- Force-Generating Mechanism of Axonemal Dynein in Solo and Ensemble.International journal of molecular sciences · 2020Review
- Stochastic Model of T Cell Repolarization during Target Elimination I.Biophysical journal · 2020Article
- Self-organized networks: Darwinian evolution of dynein rings, stalks, and stalk heads.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Current status and issues of protein solution biophysics-Session 1SDP.Biophysical reviews · 2020Article
- Cryo-EM of dynein microtubule-binding domains shows how an axonemal dynein distorts the microtubule.eLife · 2019Article
- Article
- DYNC1I1 Promotes the Proliferation and Migration of Gastric Cancer by Up-Regulating IL-6 Expression.Frontiers in oncology · 2019Article
- Step Sizes and Rate Constants of Single-headed Cytoplasmic Dynein Measured with Optical Tweezers.Scientific reports · 2018Article
- Molecular mechanisms of the interhead coordination by interhead tension in cytoplasmic dyneins.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
Abstract
Cytoplasmic dynein is a dimeric AAA(+) motor protein that performs critical roles in eukaryotic cells by moving along microtubules using ATP. Here using cryo-electron microscopy we directly observe the structure of Dictyostelium discoideum dynein dimers on microtubules at near-physiological ATP concentrations. They display remarkable flexibility at a hinge close to the microtubule binding domain (the stalkhead) producing a wide range of head positions. About half the molecules have the two heads separated from one another, with both leading and trailing motors attached to the microtubule. The other half have the two heads and stalks closely superposed in a front-to-back arrangement of the AAA(+) rings, suggesting specific contact between the heads. All stalks point towards the microtubule minus end. Mean stalk angles depend on the separation between their stalkheads, which allows estimation of inter-head tension. These findings provide a structural framework for understanding dynein's directionality and unusual stepping behaviour.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.