ArticleFEBS letters2015
Primary processing of CRISPR RNA by the endonuclease Cas6 in Staphylococcus epidermidis.
Article in FEBS letters, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 14 citations in OpenAlex.
- Article
- Characterization ofmSphere · 2024Article
- Comprehensive Analysis of CRISPR-Cas Systems and Their Influence on Antibiotic Resistance inBioinformatics and biology insights · 2024Article
- Identification and Characterization of the CRISPR/Cas System inFrontiers in microbiology · 2021Article
- Detection and characterization of clustered regularly interspaced short palindromic repeat-associated endoribonuclease gene variants inVeterinary world · 2019Article
- Article
- Study the Features of 57 Confirmed CRISPR Loci in 38 Strains ofFrontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In many bacteria and archaea, an adaptive immune system (CRISPR-Cas) provides immunity against foreign genetic elements. This system uses CRISPR RNAs (crRNAs) derived from the CRISPR array, along with CRISPR-associated (Cas) proteins, to target foreign nucleic acids. In most CRISPR systems, endonucleolytic processing of crRNA precursors (pre-crRNAs) is essential for the pathway. Here we study the Cas6 endonuclease responsible for crRNA processing in the Type III-A CRISPR-Cas system from Staphylococcus epidermidis RP62a, a model for Type III-A CRISPR-Cas systems, and define substrate requirements for SeCas6 activity. We find that SeCas6 is necessary and sufficient for full-length crRNA biogenesis in vitro, and that it relies on both sequence and stem-loop structure in the 3' half of the CRISPR repeat for recognition and processing.
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Registered trials
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