Evidence map›Paper›PMID 26363123›Full record

ArticleJournal of invertebrate pathology2017

Reverse transcription loop-mediated isothermal amplification for rapid and quantitative assay of covert mortality nodavirus in shrimp.

Qingli Zhang, Shuang Liu, Haolin Yang, Luoluo Zhu, Xiaoyuan Wan, Xiaoping Li, Jie Huang

Open access · bronzeAbstract read
In one paragraph

Article in Journal of invertebrate pathology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.2field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 23 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Qingli ZhangKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute (YSFRI), Chinese Academy of Fishery Sciences (CAFS), Qingdao 266071, China; National Laboratory for Marine Science and Technology, Qingdao 266071, China; Shanghai Ocean University, Shanghai 201306, China. Electronic address: zhangql@live.com.
Shuang LiuKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute (YSFRI), Chinese Academy of Fishery Sciences (CAFS), Qingdao 266071, China; Shanghai Ocean University, Shanghai 201306, China. Electronic address: liushuang8@163.com.
Haolin YangKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute (YSFRI), Chinese Academy of Fishery Sciences (CAFS), Qingdao 266071, China; Shanghai Ocean University, Shanghai 201306, China. Electronic address: yanghlwin@163.com.
Luoluo ZhuKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute (YSFRI), Chinese Academy of Fishery Sciences (CAFS), Qingdao 266071, China; Shanghai Ocean University, Shanghai 201306, China. Electronic address: zhuluoluo666@163.com.
Xiaoyuan WanKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute (YSFRI), Chinese Academy of Fishery Sciences (CAFS), Qingdao 266071, China; National Laboratory for Marine Science and Technology, Qingdao 266071, China. Electronic address: wanxy@ysfri.ac.cn.
Xiaoping LiKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute (YSFRI), Chinese Academy of Fishery Sciences (CAFS), Qingdao 266071, China; Shanghai Ocean University, Shanghai 201306, China.
Jie HuangKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute (YSFRI), Chinese Academy of Fishery Sciences (CAFS), Qingdao 266071, China; National Laboratory for Marine Science and Technology, Qingdao 266071, China; Shanghai Ocean University, Shanghai 201306, China. Electronic address: huangjie@ysfri.ac.cn.
Shanghai Ocean University · CNChinese Academy of Fishery Sciences · CNQingdao National Laboratory for Marine Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A disease known as covert mortality disease has become an increasing problem in the shrimp farming industry in recent years in China and several countries of Southeast Asia, leading to serious losses in production. Litopenaeus vannamei (also known as Pacific white shrimp) is affected by this disease that leads to a range of clinical symptoms including hepatopancreas atrophy and necrosis, soft shell, slow growth, and abdominal muscle whitening and necrosis in the acute stage of disease. A new nodavirus, termed covert mortality nodavirus (CMNV), has been shown to be the etiological agent. In this study, we report a sensitive and specific real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid and quantitative detection of CMNV. The optimal conditions for this newly developed RT-LAMP reaction were found to be 6mM MgCl

Indexed as

AnimalsNodaviridaeNucleic Acid Amplification TechniquesPenaeidaeReverse TranscriptionRNA Virus InfectionsViral LoadCovert mortality nodavirusLitopenaeus vannameiReverse transcription loop-mediated isothermal amplificationRT-PCRShrimp

Identifiers

PMID26363123
PMCPMC7127608
OpenAlexW2210072825

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.