ArticleApplied and environmental microbiology2015
A single mutation in the gene responsible for the mucoid phenotype of Bifidobacterium animalis subsp. lactis confers surface and functional characteristics.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Enhanced editing ofApplied and environmental microbiology · 2026Article
- A CRISPRi Gene Regulation System for Bifidobacteria.Microbial biotechnology · 2025Article
- An improved temperature-sensitive shuttle vector system for scarless gene deletion in human-gut-associatediScience · 2024Article
- Review
- Structural analysis and prebiotic activity of exopolysaccharide produced by probiotic strainFood science and biotechnology · 2023Article
- Genomic and epigenetic landscapes drive CRISPR-based genome editing inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Proposed Framework to Identify Dispensable and Essential Functions in Bifidobacteria: Case Study of Bifidobacterium breve UCC2003 as a Prototype of Its Genus.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Genetic tools for the development of recombinant lactic acid bacteria.Microbial cell factories · 2021Review
- Detection, Isolation, and Purification of Bifidobacterial Exopolysaccharides.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Evaluation of anFrontiers in microbiology · 2021Article
- Broad Purpose Vector for Site-Directed Insertional Mutagenesis inFrontiers in microbiology · 2021Article
- The Possible Link Between Manufacturing and Probiotic Efficacy; a Molecular Point of View onFrontiers in microbiology · 2021Review
- Selection of Exopolysaccharide-ProducingMicroorganisms · 2020Article
- Molecules Produced by Probiotics and Intestinal Microorganisms with Immunomodulatory Activity.Nutrients · 2020Review
- Exopolysaccharide ProducingFrontiers in microbiology · 2020Article
- Genome editing using the endogenous type I CRISPR-Cas system inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Specific properties of probiotic strains: relevance and benefits for the host.The EPMA journal · 2018Article
- Interactions of Surface Exopolysaccharides FromFrontiers in microbiology · 2018Review
- The essential genomic landscape of the commensal Bifidobacterium breve UCC2003.Scientific reports · 2017Article
- Bifidobacteria and Their Molecular Communication with the Immune System.Frontiers in microbiology · 2017Review
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14 authors.
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Abstract
Exopolysaccharides (EPS) are extracellular carbohydrate polymers synthesized by a large variety of bacteria. Their physiological functions have been extensively studied, but many of their roles have not yet been elucidated. We have sequenced the genomes of two isogenic strains of Bifidobacterium animalis subsp. lactis that differ in their EPS-producing phenotype. The original strain displays a nonmucoid appearance, and the mutant derived thereof has acquired a mucoid phenotype. The sequence analysis of their genomes revealed a nonsynonymous mutation in the gene Balat_1410, putatively involved in the elongation of the EPS chain. By comparing a strain from which this gene had been deleted with strains containing the wild-type and mutated genes, we were able to show that each strain displays different cell surface characteristics. The mucoid EPS synthesized by the strain harboring the mutation in Balat_1410 provided higher resistance to gastrointestinal conditions and increased the capability for adhesion to human enterocytes. In addition, the cytokine profiles of human peripheral blood mononuclear cells and ex vivo colon tissues suggest that the mucoid strain could have higher anti-inflammatory activity. Our findings provide relevant data on the function of Balat_1410 and reveal that the mucoid phenotype is able to alter some of the most relevant functional properties of the cells.
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