Evidence map›Paper›PMID 26362676›Full record

ArticleEndocrine-related cancer2015

Glucose-deprivation increases thyroid cancer cells sensitivity to metformin.

Athanasios Bikas, Kirk Jensen, Aneeta Patel, John Costello, Dennis McDaniel, Joanna Klubo-Gwiezdzinska, Olexander Larin, Victoria Hoperia, Kenneth D Burman, Lisa Boyle and 2 more

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Endocrine-related cancer, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 57 citations in OpenAlex.

  1. Review
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  3. Metabolic Reprogramming in Thyroid Cancer.Endocrinology and metabolism (Seoul, Korea) · 2024
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Athanasios BikasDivision of EndocrinologyDepartment of Medicine, Medstar Washington Hospital Center, 110 Irving Street Northwest, Washington, District of Columbia 20010, USADepartment of PediatricsUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USABiomedical Instrumental CenterUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USACenter for Endocrine SurgeryKiev, UkraineDepartment of SurgeryMedstar Georgetown University Hospital, 3800 Reservoir Road, Washington, District of Columbia 20007, USA.
Kirk JensenDivision of EndocrinologyDepartment of Medicine, Medstar Washington Hospital Center, 110 Irving Street Northwest, Washington, District of Columbia 20010, USADepartment of PediatricsUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USABiomedical Instrumental CenterUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USACenter for Endocrine SurgeryKiev, UkraineDepartment of SurgeryMedstar Georgetown University Hospital, 3800 Reservoir Road, Washington, District of Columbia 20007, USA.
Aneeta PatelDivision of EndocrinologyDepartment of Medicine, Medstar Washington Hospital Center, 110 Irving Street Northwest, Washington, District of Columbia 20010, USADepartment of PediatricsUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USABiomedical Instrumental CenterUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USACenter for Endocrine SurgeryKiev, UkraineDepartment of SurgeryMedstar Georgetown University Hospital, 3800 Reservoir Road, Washington, District of Columbia 20007, USA.
John CostelloDivision of EndocrinologyDepartment of Medicine, Medstar Washington Hospital Center, 110 Irving Street Northwest, Washington, District of Columbia 20010, USADepartment of PediatricsUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USABiomedical Instrumental CenterUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USACenter for Endocrine SurgeryKiev, UkraineDepartment of SurgeryMedstar Georgetown University Hospital, 3800 Reservoir Road, Washington, District of Columbia 20007, USA.
Dennis McDanielDivision of EndocrinologyDepartment of Medicine, Medstar Washington Hospital Center, 110 Irving Street Northwest, Washington, District of Columbia 20010, USADepartment of PediatricsUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USABiomedical Instrumental CenterUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USACenter for Endocrine SurgeryKiev, UkraineDepartment of SurgeryMedstar Georgetown University Hospital, 3800 Reservoir Road, Washington, District of Columbia 20007, USA.
Joanna Klubo-GwiezdzinskaDivision of EndocrinologyDepartment of Medicine, Medstar Washington Hospital Center, 110 Irving Street Northwest, Washington, District of Columbia 20010, USADepartment of PediatricsUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USABiomedical Instrumental CenterUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USACenter for Endocrine SurgeryKiev, UkraineDepartment of SurgeryMedstar Georgetown University Hospital, 3800 Reservoir Road, Washington, District of Columbia 20007, USA.
Olexander LarinDivision of EndocrinologyDepartment of Medicine, Medstar Washington Hospital Center, 110 Irving Street Northwest, Washington, District of Columbia 20010, USADepartment of PediatricsUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USABiomedical Instrumental CenterUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USACenter for Endocrine SurgeryKiev, UkraineDepartment of SurgeryMedstar Georgetown University Hospital, 3800 Reservoir Road, Washington, District of Columbia 20007, USA.
Victoria HoperiaDivision of EndocrinologyDepartment of Medicine, Medstar Washington Hospital Center, 110 Irving Street Northwest, Washington, District of Columbia 20010, USADepartment of PediatricsUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USABiomedical Instrumental CenterUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USACenter for Endocrine SurgeryKiev, UkraineDepartment of SurgeryMedstar Georgetown University Hospital, 3800 Reservoir Road, Washington, District of Columbia 20007, USA.
Kenneth D BurmanDivision of EndocrinologyDepartment of Medicine, Medstar Washington Hospital Center, 110 Irving Street Northwest, Washington, District of Columbia 20010, USADepartment of PediatricsUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USABiomedical Instrumental CenterUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USACenter for Endocrine SurgeryKiev, UkraineDepartment of SurgeryMedstar Georgetown University Hospital, 3800 Reservoir Road, Washington, District of Columbia 20007, USA.
Lisa BoyleDivision of EndocrinologyDepartment of Medicine, Medstar Washington Hospital Center, 110 Irving Street Northwest, Washington, District of Columbia 20010, USADepartment of PediatricsUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USABiomedical Instrumental CenterUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USACenter for Endocrine SurgeryKiev, UkraineDepartment of SurgeryMedstar Georgetown University Hospital, 3800 Reservoir Road, Washington, District of Columbia 20007, USA.
Leonard WartofskyDivision of EndocrinologyDepartment of Medicine, Medstar Washington Hospital Center, 110 Irving Street Northwest, Washington, District of Columbia 20010, USADepartment of PediatricsUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USABiomedical Instrumental CenterUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USACenter for Endocrine SurgeryKiev, UkraineDepartment of SurgeryMedstar Georgetown University Hospital, 3800 Reservoir Road, Washington, District of Columbia 20007, USA.
Vasyl VaskoDivision of EndocrinologyDepartment of Medicine, Medstar Washington Hospital Center, 110 Irving Street Northwest, Washington, District of Columbia 20010, USADepartment of PediatricsUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USABiomedical Instrumental CenterUniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, Maryland 20814-4712, USACenter for Endocrine SurgeryKiev, UkraineDepartment of SurgeryMedstar Georgetown University Hospital, 3800 Reservoir Road, Washington, District of Columbia 20007, USA vasyl.vasko.ctr@usuhs.edu.
Uniformed Services University of the Health Sciences · USGeorgetown University · USMedStar Washington Hospital Center · US

Funding

NoneU2GPS000658 · PS · NATIONAL AIDS COUNCIL · PI MAGURE, TAPUWA · 2006 to 2007
$200k
6 · The paper itself

Abstract

Metformin inhibits thyroid cancer cell growth. We sought to determine if variable glucose concentrations in medium alter the anti-cancer efficacy of metformin. Thyroid cancer cells (FTC133 and BCPAP) were cultured in high-glucose (20 mM) and low-glucose (5 mM) medium before treatment with metformin. Cell viability and apoptosis assays were performed. Expression of glycolytic genes was examined by real-time PCR, western blot, and immunostaining. Metformin inhibited cellular proliferation in high-glucose medium and induced cell death in low-glucose medium. In low-, but not in high-glucose medium, metformin induced endoplasmic reticulum stress, autophagy, and oncosis. At micromolar concentrations, metformin induced phosphorylation of AMP-activated protein kinase and blocked p-pS6 in low-glucose medium. Metformin increased the rate of glucose consumption from the medium and prompted medium acidification. Medium supplementation with glucose reversed metformin-inducible morphological changes. Treatment with an inhibitor of glycolysis (2-deoxy-d-glucose (2-DG)) increased thyroid cancer cell sensitivity to metformin. The combination of 2-DG with metformin led to cell death. Thyroid cancer cell lines were characterized by over-expression of glycolytic genes, and metformin decreased the protein level of pyruvate kinase muscle 2 (PKM2). PKM2 expression was detected in recurrent thyroid cancer tissue samples. In conclusion, we have demonstrated that the glucose concentration in the cellular milieu is a factor modulating metformin's anti-cancer activity. These data suggest that the combination of metformin with inhibitors of glycolysis could represent a new strategy for the treatment of thyroid cancer.

Indexed as

Adenocarcinoma, FollicularAMP-Activated Protein KinasesApoptosisCarcinoma, PapillaryCarrier ProteinsCaspasesCell DivisionCell Line, TumorCulture MediaDeoxyglucoseDrug Screening Assays, AntitumorDrug SynergismEndoplasmic ReticulumEndoplasmic Reticulum Chaperone BiPEnzyme ActivationGene Expression ProfilingAMP-Activated Protein KinasesCarrier ProteinsCaspasesCulture MediaDeoxyglucoseEndoplasmic Reticulum Chaperone BiPGlucoseHeat-Shock ProteinsMembrane ProteinsMetforminNeoplasm ProteinsThyroid Hormone-Binding ProteinsThyroid Hormonescell signalingglucosemetforminoncosisthyroid cancer

Identifiers

PMID26362676
OpenAlexW2246558836

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.