ArticlePloS one2015
A 3D-Printed Oxygen Control Insert for a 24-Well Plate.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Profiling paracrine interactions between hypoxic and normoxic skeletal muscle tissue in a microphysiological system fabricated from 3D printed components.Lab on a chip · 2025Article
- Continuous flow delivery system for the perfusion of scaffold-based 3D cultures.Lab on a chip · 2024Article
- Additively manufactured customized microhelix motors' bursting motion in mesoscopic tubes for vessel declogging.RSC advances · 2024Article
- Snap-on Adaptor for Microtiter Plates to Enable Continuous-Flow Microfluidic Screening and Harvesting of Crystalline Materials.ACS omega · 2023Article
- Emulating clinical pressure waveforms in cell culture using an Arduino-controlled millifluidic 3D-printed platform for 96-well plates.Lab on a chip · 2023Article
- Experimental Models to Study End-Organ Morbidity in Sleep Apnea: Lessons Learned and Future Directions.International journal of molecular sciences · 2022Review
- A Comprehensive Assessment on the Pivotal Role of Hydrogels in Scaffold-Based Bioprinting.Gels (Basel, Switzerland) · 2022Review
- A multiwell plate-based system for toxicity screening under multiple static or cycling oxygen environments.Scientific reports · 2021Article
- 96-Well Oxygen Control Using a 3D-Printed Device.Analytical chemistry · 2021Article
- Flow-Encoded Oxygen Control to Track the Time-Dependence of Molecular Changes Induced by Static or Cycling Hypoxia.Analytical chemistry · 2019Article
- Hypoxic niches are endowed with a protumorigenic mechanism that supersedes the protective function of PTEN.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Digital Manufacturing for Microfluidics.Annual review of biomedical engineering · 2019Review
- 3D-printed miniaturized fluidic tools in chemistry and biology.Trends in analytical chemistry : TRAC · 2018Article
- Open Design 3D-Printable Adjustable Micropipette that Meets the ISO Standard for Accuracy.Micromachines · 2018Article
- Noninvasive Absolute Electron Paramagnetic Resonance Oxygen Imaging for the Assessment of Tissue Graft Oxygenation.Tissue engineering. Part C, Methods · 2018Article
- Gas Partial Pressure in Cultured Cells: Patho-Physiological Importance and Methodological Approaches.Frontiers in physiology · 2018Review
- A Microdevice Platform Recapitulating Hypoxic Tumor Microenvironments.Scientific reports · 2017Article
- A microfluidic oxygen gradient demonstrates differential activation of the hypoxia-regulated transcription factors HIF-1α and HIF-2α.Integrative biology : quantitative biosciences from nano to macro · 2017Article
- Microfluidic on-chip biomimicry for 3D cell culture: a fit-for-purpose investigation from the end user standpoint.Future science OA · 2017Review
- BaroFuse, a novel pressure-driven, adjustable-throughput perfusion system for tissue maintenance and assessment.Heliyon · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
3D printing has emerged as a method for directly printing complete microfluidic devices, although printing materials have been limited to oxygen-impermeable materials. We demonstrate the addition of gas permeable PDMS (Polydimethylsiloxane) membranes to 3D-printed microfluidic devices as a means to enable oxygen control cell culture studies. The incorporation of a 3D-printed device and gas-permeable membranes was demonstrated on a 24-well oxygen control device for standard multiwell plates. The direct printing allows integrated distribution channels and device geometries not possible with traditional planar lithography. With this device, four different oxygen conditions were able to be controlled, and six wells were maintained under each oxygen condition. We demonstrate enhanced transcription of the gene VEGFA (vascular endothelial growth factor A) with decreasing oxygen levels in human lung adenocarcinoma cells. This is the first 3D-printed device incorporating gas permeable membranes to facilitate oxygen control in cell culture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.