ArticlePLoS genetics2015
Synergistic and Dose-Controlled Regulation of Cellulase Gene Expression in Penicillium oxalicum.
Article in PLoS genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed, 200 citations in OpenAlex.
- The carbohydrate utilization regulator Cbr1 coordinates nutrient-specific gene activation with selective carbon catabolite repression in a basidiomycete yeast.PLoS biology · 2026Article
- Modifying the upstream open reading frames of cellulase gene enhances cellulase production inSynthetic and systems biotechnology · 2026Article
- Transcription factor MtCLR-2 regulates cellulase production via direct modulation of Mtegl2 and Mtbgl1 expression in Myceliophthora thermophila.Microbial cell factories · 2026Article
- Competitive binding of TeXlnR and TeAmyR regulates cellulase production inSynthetic and systems biotechnology · 2026Article
- The global regulator LaeA controls expression of genes involved in diverse primary and secondary metabolites biosynthesis and their secretion in fungi.Frontiers in microbiology · 2026Review
- A GTPase activating protein Polrg1 regulates the development and cellulase expression of Penicillium oxalicum.World journal of microbiology & biotechnology · 2025Article
- Screening, Characterization and Comparison of Endoglucanases/Xylanases from Thermophilic Fungi: AInternational journal of molecular sciences · 2025Article
- Improvement of β-Xylosidase and Endoxylanase Activities in Talaromyces amestolkiae by Genetic Manipulation of the Transcriptional Activator XlnR.Microbial biotechnology · 2025Article
- Discovery of a novel translation-machinery-associated protein that positively correlates with cellulase production.Biotechnology for biofuels and bioproducts · 2025Article
- F-box protein Fbx23 acts as a transcriptional coactivator to recognize and activate transcription factor Ace1.PLoS genetics · 2025Article
- Regulation of genes encoding polysaccharide-degrading enzymes in Penicillium.Applied microbiology and biotechnology · 2024Review
- Developing endophytic Penicillium oxalicum as a source of lignocellulolytic enzymes for enhanced hydrolysis of biorefinery relevant pretreated rice straw.Bioprocess and biosystems engineering · 2024Article
- The CBS/HCommunications biology · 2024Article
- The protein methyltransferase TrSAM inhibits cellulase gene expression by interacting with the negative regulator ACE1 in Trichoderma reesei.Communications biology · 2024Article
- Improving gene set enrichment analysis (GSEA) by using regulation directionality.Microbiology spectrum · 2024Article
- Calcium signaling positively regulates cellulase translation and secretion in a Clr-2-overexpressing, catabolically derepressed strain of Penicillium funiculosum.Biotechnology for biofuels and bioproducts · 2024Article
- Functional Study of cAMP-Dependent Protein Kinase A inJournal of fungi (Basel, Switzerland) · 2023Article
- Overexpression of the Transcription Factor Azf1 Reveals Novel Regulatory Functions and Impacts β-Glucosidase Production inJournal of fungi (Basel, Switzerland) · 2023Article
- Effect of the res2 transcription factor gene deletion on protein secretion and stress response in the hyperproducer strain Trichoderma reesei Rut-C30.BMC microbiology · 2023Article
- The transcriptional factor Clr-5 is involved in cellulose degradation through regulation of amino acid metabolism in Neurospora crassa.BMC biotechnology · 2023Article
27 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Filamentous fungus Penicillium oxalicum produces diverse lignocellulolytic enzymes, which are regulated by the combinations of many transcription factors. Here, a single-gene disruptant library for 470 transcription factors was constructed and systematically screened for cellulase production. Twenty transcription factors (including ClrB, CreA, XlnR, Ace1, AmyR, and 15 unknown proteins) were identified to play putative roles in the activation or repression of cellulase synthesis. Most of these regulators have not been characterized in any fungi before. We identified the ClrB, CreA, XlnR, and AmyR transcription factors as critical dose-dependent regulators of cellulase expression, the core regulons of which were identified by analyzing several transcriptomes and/or secretomes. Synergistic and additive modes of combinatorial control of each cellulase gene by these regulatory factors were achieved, and cellulase expression was fine-tuned in a proper and controlled manner. With one of these targets, the expression of the major intracellular β-glucosidase Bgl2 was found to be dependent on ClrB. The Bgl2-deficient background resulted in a substantial gene activation by ClrB and proved to be closely correlated with the relief of repression mediated by CreA and AmyR during cellulase induction. Our results also signify that probing the synergistic and dose-controlled regulation mechanisms of cellulolytic regulators and using it for reconstruction of expression regulation network (RERN) may be a promising strategy for cellulolytic fungi to develop enzyme hyper-producers. Based on our data, ClrB was identified as focal point for the synergistic activation regulation of cellulase expression by integrating cellulolytic regulators and their target genes, which refined our understanding of transcriptional-regulatory network as a "seesaw model" in which the coordinated regulation of cellulolytic genes is established by counteracting activators and repressors.
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