Methods for analyzing the role of phospholipase A₂ enzymes in endosome membrane tubule formation.

Danielle N Kalkofen, Paul de Figueiredo, William J Brown

Abstract read

In one paragraph

Article in Methods in cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

The Synthetic Cannabinoid WIN55,212-2 Can Disrupt the Golgi Apparatus Independent of Cannabinoid Receptor-1.Molecular pharmacology · 2022
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Danielle N KalkofenDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA.

Paul de FigueiredoDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA; Department of Microbial Pathogenesis and Immunology, Norman Borlaug Center, Texas A&M University, College Station, TX, USA.

William J BrownDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA.

Funding

Role of Phospholipid Remodeling in Secretion and Golgi Function in Mammalian CellR01GM101027 · NIGMS · CORNELL UNIVERSITY · PI BROWN, WILLIAM J · 2013 to 2016

$1.2M

NIGMS NIH HHS GM 101027NIGMS NIH HHS R01 GM101027

6 · The paper itself

Abstract

Cargo export from mammalian endosomal compartments often involves membrane tubules, into which soluble and membrane-bound cargos are segregated for subsequent intracellular transport. These membrane tubules are highly dynamic and their formation is mediated by a variety of endosome-associated proteins. However, little is known about how these membrane tubules are temporally or spatially regulated, so other tubule-associated proteins are likely to be discovered and analyzed. Therefore, methods to examine the biogenesis and regulation of endosome membrane tubules will prove to be valuable for cell biologists. In this chapter, we describe methods for studying this process using both cell-free, in vitro reconstitution assays, and in vivo image analysis tools.

Indexed as

AnimalsBrainCattleEndosomesHeLa CellsHumansIntracellular MembranesMiceMicroscopy, Electron, TransmissionPhospholipases A2Protein TransportRatsRAW 264.7 CellsPhospholipases A2Digital image analysisEndosome tubulesIn vitro reconstitutionPhospholipase A(2)(PLA(2)) enzymesTransferrin trafficking

Identifiers

PMID26360034

DOI10.1016/bs.mcb.2015.05.005

PMCPMC4876852

What OpenQuestion holds

Textmetadata

LicenceTDM

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.