ArticleMethods in cell biology2015
Biochemical methods for studying kinetic regulation of Arf1 activation by Sec7.
Article in Methods in cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Sec7 regulatory domains scaffold autoinhibited and active conformations.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The Arf-GAP Age2 localizes to the late-Golgi via a conserved amphipathic helix.Molecular biology of the cell · 2023Article
- The Arf-GAP Age2 localizes to the late-Golgi via a conserved amphipathic helix.bioRxiv : the preprint server for biology · 2023Article
- Structural basis for activation of Arf1 at the Golgi complex.Cell reports · 2022Article
- Structural basis for the initiation of COPII vesicle biogenesis.Structure (London, England : 1993) · 2021Article
- Arf1 directly recruits the Pik1-Frq1 PI4K complex to regulate the final stages of Golgi maturation.Molecular biology of the cell · 2021Article
- Yeast Rgd3 is a phospho-regulated F-BAR-containing RhoGAP involved in the regulation of Rho3 distribution and cell morphology.Molecular biology of the cell · 2020Article
- A Steric Gating Mechanism Dictates the Substrate Specificity of a Rab-GEF.Developmental cell · 2019Article
- The HUS box is required for allosteric regulation of the Sec7 Arf-GEF.The Journal of biological chemistry · 2018Article
- The two TRAPP complexes of metazoans have distinct roles and act on different Rab GTPases.The Journal of cell biology · 2018Article
- Regulation of Arf activation occurs via distinct mechanisms at early and late Golgi compartments.Molecular biology of the cell · 2017Article
- GTPase cross talk regulates TRAPPII activation of Rab11 homologues during vesicle biogenesis.The Journal of cell biology · 2016Article
- The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase.eLife · 2016Article
- Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor.Methods in molecular biology (Clifton, N.J.) · 2016Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The ADP ribosylation factor (Arf) family of small guanosine triphosphatases (GTPases) regulates vesicular transport at several locations within the cell, and is in turn regulated by guanine nucleotide exchange factors (GEFs) via a conserved catalytic domain, termed the Sec7 domain. The catalytic activity of the Sec7 domain is well characterized in the context of a few GEFs acting at the periphery of the cell. This chapter describes the techniques used to extend the biochemical analysis of activity to the much larger GEFs acting on the Arf family in the core secretory pathway, using the activity of Saccharomyces cerevisiae Sec7 on Arf1, regulating export from the trans-Golgi network, as a model. The complete methods for purification to near homogeneity of all proteins required, including several Sec7 constructs and multiple relevant small GTPases, are detailed. These are followed by methods for the quantification of the nucleotide exchange activity of Sec7 in a physiologically relevant context, including modifications required to dissect the signal integration functions of Sec7 as an effector of several other small GTPases, and methods for identifying stable Sec7-small GTPase interactions in the presence of membranes. These techniques may be extended to the analysis of similar members of the Sec7 GEF subfamily in other species and acting elsewhere in the secretory pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.