ArticleJournal of cell science2015
Two-color fluorescent analysis of connexin 36 turnover: relationship to functional plasticity.
Article in Journal of cell science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 56 citations in OpenAlex.
- Article
- Uncovering the electrical synapse proteome in retinal neurons via in vivo proximity labeling.eLife · 2026Article
- Diminished gap junction coupling under diabetogenic conditions does not drive loss of functional β-cell subpopulations.The Journal of general physiology · 2026Article
- Single-cell synaptome mapping of endogenous protein subpopulations in mammalian brain.Nature communications · 2025Article
- Characterizing ER Retention Defects of PDZ Binding Deficient Cx36 Mutants Using Confocal Microscopy.Bio-protocol · 2024Article
- Future avenues inLearning & memory (Cold Spring Harbor, N.Y.) · 2024Review
- Design of a palette of SNAP-tag mimics of fluorescent proteins and their use as cell reporters.Cell discovery · 2023Article
- Neurobeachin controls the asymmetric subcellular distribution of electrical synapse proteins.Current biology : CB · 2023Article
- The Amino Terminal Domain and Modulation of Connexin36 Gap Junction Channels by Intracellular Magnesium Ions.Frontiers in physiology · 2022Article
- Article
- The SNARE regulator Complexin3 is a target of the cone circadian clock.The Journal of comparative neurology · 2021Article
- Altered Expression of GJD2 Messenger RNA and the Coded Protein Connexin 36 in Negative Lens-induced Myopia of Guinea Pigs.Optometry and vision science : official publication of the American Academy of Optometry · 2020Article
- Phosphorylation of Connexin36 near the C-terminus switches binding affinities for PDZ-domain and 14-3-3 proteins in vitro.Scientific reports · 2020Article
- The dynamic Nexus: gap junctions control protein localization and mobility in distinct and surprising ways.Scientific reports · 2020Article
- Endocytosis of Connexin 36 is Mediated by Interaction with Caveolin-1.International journal of molecular sciences · 2020Article
- A non-fluorescent HaloTag blocker for improved measurement and visualization of protein synthesis in living cells.F1000Research · 2020Article
- Understanding the Molecular and Cell Biological Mechanisms of Electrical Synapse Formation.Frontiers in neuroanatomy · 2020Article
- A robust and economical pulse-chase protocol to measure the turnover of HaloTag fusion proteins.The Journal of biological chemistry · 2019Article
- Tubulin-Dependent Transport of Connexin-36 Potentiates the Size and Strength of Electrical Synapses.Cells · 2019Article
- Connexin-36 distribution and layer-specific topography in the cat retina.Brain structure & function · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Gap junctions formed of connexin 36 (Cx36, also known as Gjd2) show tremendous functional plasticity on several time scales. Changes in connexin phosphorylation modify coupling in minutes through an order of magnitude, but recent studies also imply involvement of connexin turnover in regulating cell-cell communication. We utilized Cx36 with an internal HaloTag to study Cx36 turnover and trafficking in cultured cells. Irreversible, covalent pulse-chase labeling with fluorescent HaloTag ligands allowed clear discrimination of newly formed and pre-existing Cx36. Cx36 in junctional plaques turned over with a half-life of 3.1 h, and the turnover rate was unchanged by manipulations of protein kinase A (PKA) activity. In contrast, changes in PKA activity altered coupling within 20 min. New Cx36 in cargo vesicles was added directly to existing gap junctions and newly made Cx36 was not confined to points of addition, but diffused throughout existing gap junctions. Existing connexins also diffused into photobleached areas with a half-time of less than 2 s. In conclusion, studies of Cx36-HaloTag revealed novel features of connexin trafficking and demonstrated that phosphorylation-based changes in coupling occur on a different time scale than turnover.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.