ArticleScientific reports2015
In-depth proteomic analysis of Varroa destructor: Detection of DWV-complex, ABPV, VdMLV and honeybee proteins in the mite.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Interactions of husbandry, landscape, and immunity in regulating viral loads for managed honey bees.Biology open · 2025Article
- Parasitic Mites of Honey Bees (Apis Spp.): A Detailed Review of Varroa destructor in Parasitism, Pathogen Transmission and its Management.Acta parasitologica · 2025Review
- Salivary Cystatin-L2-like ofAnimals : an open access journal from MDPI · 2023Article
- The Acari Hypothesis, II: Interspecies Operability of Pattern Recognition Receptors.Pathogens (Basel, Switzerland) · 2021Article
- Direct Evidence for Infection ofJournal of virology · 2021Article
- The novel insecticides flupyradifurone and sulfoxaflor do not act synergistically with viral pathogens in reducing honey bee (Apis mellifera) survival but sulfoxaflor modulates host immunocompetence.Microbial biotechnology · 2021Article
- Mode of Transmission Determines the Virulence ofViruses · 2020Article
- Green Bees: Reverse Genetic Analysis of Deformed Wing Virus Transmission, Replication, and Tropism.Viruses · 2020Article
- Contrasting impacts of a novel specialist vector on multihost viral pathogen epidemiology in wild and managed bees.Molecular ecology · 2020Article
- Honey Bee Suppresses the Parasitic Mite Vitellogenin by Antimicrobial Peptide.Frontiers in microbiology · 2020Article
- Phytoseiid predatory mites can disperse entomopathogenic fungi to prey patches.Scientific reports · 2019Article
- Varroa destructor parasitism has a greater effect on proteome changes than the deformed wing virus and activates TGF-β signaling pathways.Scientific reports · 2019Article
- Insights into the metabolism and behaviour of Varroa destructor mites from analysis of their waste excretions.Parasitology · 2019Article
- Article
- Transcriptome profiling of the honeybee parasite Varroa destructor provides new biological insights into the mite adult life cycle.BMC genomics · 2018Article
- Using an in vitro system for maintaining Varroa destructor mites on Apis mellifera pupae as hosts: studies of mite longevity and feeding behavior.Experimental & applied acarology · 2018Article
- Mitigating effects of pollen during paraquat exposure on gene expression and pathogen prevalence in Apis mellifera L.Ecotoxicology (London, England) · 2018Article
- AMolecular & cellular proteomics : MCP · 2017Article
- Review
- Changes in the Bacteriome of Honey Bees Associated with the Parasite Varroa destructor, and Pathogens Nosema and Lotmaria passim.Microbial ecology · 2017Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated pathogens in the parasitic honeybee mite Varroa destructor using nanoLC-MS/MS (TripleTOF) and 2D-E-MS/MS proteomics approaches supplemented with affinity-chromatography to concentrate trace target proteins. Peptides were detected from the currently uncharacterized Varroa destructor Macula-like virus (VdMLV), the deformed wing virus (DWV)-complex and the acute bee paralysis virus (ABPV). Peptide alignments revealed detection of complete structural DWV-complex block VP2-VP1-VP3, VDV-1 helicase and single-amino-acid substitution A/K/Q in VP1, the ABPV structural block VP1-VP4-VP2-VP3 including uncleaved VP4/VP2, and VdMLV coat protein. Isoforms of viral structural proteins of highest abundance were localized via 2D-E. The presence of all types of capsid/coat proteins of a particular virus suggested the presence of virions in Varroa. Also, matches between the MWs of viral structural proteins on 2D-E and their theoretical MWs indicated that viruses were not digested. The absence/scarce detection of non-structural proteins compared with high-abundance structural proteins suggest that the viruses did not replicate in the mite; hence, virions accumulate in the Varroa gut via hemolymph feeding. Hemolymph feeding also resulted in the detection of a variety of honeybee proteins. The advantages of MS-based proteomics for pathogen detection, false-positive pathogen detection, virus replication, posttranslational modifications, and the presence of honeybee proteins in Varroa are discussed.
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