Evidence map›Paper›PMID 26357865›Full record

ArticlePlant signaling & behavior2015

A simple method for the addition of rotenone in Arabidopsis thaliana leaves.

María V Maliandi, Sebastián P Rius, María V Busi, Diego F Gomez-Casati

Abstract read
In one paragraph

Article in Plant signaling & behavior, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

María V Maliandia IIB- INTECH; Universidad Nacional de General San Martín (UNSAM) ; Chascomús, Buenos Aires , Argentina.
Sebastián P Riusb Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET); Universidad Nacional de Rosario ; Rosario , Argentina.
María V Busib Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET); Universidad Nacional de Rosario ; Rosario , Argentina.
Diego F Gomez-Casatib Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET); Universidad Nacional de Rosario ; Rosario , Argentina.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A simple and reproducible method for the treatment of Arabidopsis thaliana leaves with rotenone is presented. Rosette leaves were incubated with rotenone and Triton X-100 for at least 15 h. Treated leaves showed increased expression of COX19 and BCS1a, 2 genes known to be induced in Arabidopsis cell cultures after rotenone treatment. Moreover, rotenone/Triton X-100 incubated leaves presented an inhibition of oxygen uptake. The simplicity of the procedure shows this methodology is useful for studying the effect of the addition of rotenone to a photosynthetic tissue in situ.

Indexed as

Cell BiologyArabidopsisArabidopsis ProteinsBotanyCell Culture TechniquesElectron Transport Complex IGenes, PlantMitochondriaMitochondrial ProteinsOctoxynolOxygenPhotosynthesisPlant LeavesRotenoneArabidopsis ProteinsElectron Transport Complex IMitochondrial ProteinsOctoxynolOM66 protein, ArabidopsisOxygenRotenoneArabidopsiscomplex Imitochondriamitochondrial respirationrotenone

Identifiers

PMID26357865
PMCPMC4883935

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.