ArticlePlant signaling & behavior2015
RNAi-mediated gene silencing of WsSGTL1 in W.somnifera affects growth and glycosylation pattern.
Article in Plant signaling & behavior, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 16 citations in OpenAlex.
- Characterization of two glycosyltransferases that modulate withanolide biosynthesis and defense in Withania somnifera.Molecular biology reports · 2025Article
- Molecular Cloning and Functional Characterization of a Sterol 3-Frontiers in plant science · 2021Article
- Fungal endophytes attune withanolide biosynthesis in Withania somnifera, prime to enhanced withanolide A content in leaves and roots.World journal of microbiology & biotechnology · 2019Article
- Third DWF1 paralog in Solanaceae, sterol ΔProceedings of the National Academy of Sciences of the United States of America · 2018Article
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5 authors at 3 institutions in 1 country.
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Abstract
Sterol glycosyltransferases (SGTs) belong to family 1 of glycosyltransferases (GTs) and are enzymes responsible for synthesis of sterol-glucosides (SGs) in many organisms. WsSGTL1 is a SGT of Withania somnifera that has been found associated with plasma membranes. However its biological function in W.somnifera is largely unknown. In the present study, we have demonstrated through RNAi silencing of WsSGTL1 gene that it performs glycosylation of withanolides and sterols resulting in glycowithanolides and glycosylated sterols respectively, and affects the growth and development of transgenic W.somnifera. For this, RNAi construct (pFGC1008-WsSGTL1) was made and genetic transformation was done by Agrobacterium tumefaciens. HPLC analysis depicts the reduction of withanoside V (the glycowithanolide of W.somnifera) and a large increase of withanolides (majorly withaferin A) content. Also, a significant decrease in level of glycosylated sterols has been observed. Hence, the obtained data provides an insight into the biological function of WsSGTL1 gene in W.somnifera.
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