ArticleGM crops & food2015
CRISPRing into the woods.
Article in GM crops & food, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Identification of poplar and spruce GDP-mannose pyrophosphorylases involved in mannan and ascorbic acid biosynthesis.Tree physiology · 2026Article
- Unraveling mannan biosynthesis in poplar and spruce: functional characterization of Cellulose Synthase-Like family A (CSLA) genes.The New phytologist · 2026Article
- Populus VariantDB v3.2 facilitates CRISPR and functional genomics research.Tree physiology · 2025Article
- A Protoplast System for CRISPR-Cas Ribonucleoprotein Delivery inPlants (Basel, Switzerland) · 2025Article
- Engineering custom morpho- and chemotypes ofFrontiers in plant science · 2023Review
- CRISPR/Cas9 and Nanotechnology Pertinence in Agricultural Crop Refinement.Frontiers in plant science · 2022Review
- Is CRISPR/Cas9 a way forward to fast-track genetic improvement in commercial palms? Prospects and limits.Frontiers in plant science · 2022Review
- Achievements and Challenges of Genomics-Assisted Breeding in Forest Trees: From Marker-Assisted Selection to Genome Editing.International journal of molecular sciences · 2021Review
- Induced Genetic Variations in Fruit Trees Using New Breeding Tools: Food Security and Climate Resilience.Plants (Basel, Switzerland) · 2021Review
- Thermophilic microbial deconstruction and conversion of natural and transgenic lignocellulose.Environmental microbiology reports · 2021Review
- Omics and CRISPR-Cas9 Approaches for Molecular Insight, Functional Gene Analysis, and Stress Tolerance Development in Crops.International journal of molecular sciences · 2021Review
- Implementing the CRISPR/Cas9 Technology inInternational journal of molecular sciences · 2020Article
- CRISPR/Cas9 Gene Editing: An Unexplored Frontier for Forest Pathology.Frontiers in plant science · 2020Review
- Sustainable bioenergy for climate mitigation: developing drought-tolerant trees and grasses.Annals of botany · 2019Article
- Vessel-Specific Reintroduction of CINNAMOYL-COA REDUCTASE1 (CCR1) in DwarfedPlant physiology · 2018Article
- Manipulation of Growth and Architectural Characteristics in Trees for Increased Woody Biomass Production.Frontiers in plant science · 2018Article
- Structural variability and niche differentiation in the rhizosphere and endosphere bacterial microbiome of field-grown poplar trees.Microbiome · 2017Article
- CRISPR/Cas9: A Practical Approach in Date Palm Genome Editing.Frontiers in plant science · 2017Review
- Recent Developments in Systems Biology and Metabolic Engineering of Plant-Microbe Interactions.Frontiers in plant science · 2016Review
- Biosynthesis and Metabolic Fate of Phenylalanine in Conifers.Frontiers in plant science · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CRISPR/Cas9 technology is a welcome breakthrough for genome editing, owing to its precision, efficiency, versatility and ease of adoption. We recently reported the first application of CRISPR/Cas9 for biallelic mutations in stably transformed Populus, extending the species range of this powerful technology to woody perennials. An underappreciated obstacle in genome editing of outcrossing species is the frequent occurrence of sequence polymorphisms that can render CRISPR/Cas9 unproductive. We discuss experimental evidence as well as genome-wide computational analysis to demonstrate the sensitivity of CRISPR/Cas9 to allelic heterozygosity, and highlight tools and strategies that can help deal with such sequence polymorphisms. With its specificity, CRISPR/Cas9 offers a less equivocal means than previous approaches for discerning functional redundancy of paralogous genes that are prevalent in plant genomes. Continuing improvements of the CRISPR/Cas9 system for multiplex genome engineering should facilitate these efforts. The paradigm shift brought about by CRISPR/Cas9 promises to accelerate not only basic research but also applied crop improvement progress.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.