Evidence map›Paper›PMID 26357517›Full record

ArticleStem cells international2015

Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In Vitro.

Klaus Neef, Philipp Treskes, Guoxing Xu, Florian Drey, Sureshkumar Perumal Srinivasan, Tomo Saric, Erastus Nembo, Judith Semmler, Filomain Nguemo, Christof Stamm and 7 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 91% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 3 institutions in 2 countries.

Klaus NeefDepartment of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, Germany ; Center for Molecular Medicine Cologne, University of Cologne, 50931 Cologne, Germany.
Philipp TreskesDepartment of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, Germany ; Center for Molecular Medicine Cologne, University of Cologne, 50931 Cologne, Germany.
Guoxing XuInstitute for Neurophysiology, University of Cologne, 50931 Cologne, Germany.
Florian DreyDepartment of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, Germany ; Center for Molecular Medicine Cologne, University of Cologne, 50931 Cologne, Germany.
Sureshkumar Perumal SrinivasanDepartment of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, Germany ; Center for Molecular Medicine Cologne, University of Cologne, 50931 Cologne, Germany.
Tomo SaricInstitute for Neurophysiology, University of Cologne, 50931 Cologne, Germany.
Erastus NemboInstitute for Neurophysiology, University of Cologne, 50931 Cologne, Germany.
Judith SemmlerInstitute for Neurophysiology, University of Cologne, 50931 Cologne, Germany.
Filomain NguemoInstitute for Neurophysiology, University of Cologne, 50931 Cologne, Germany.
Christof StammBerlin-Brandenburg Center for Regenerative Therapies, 13353 Berlin, Germany.
Douglas B CowanDepartment of Anesthesiology, Perioperative and Pain Medicine, Children's Hospital Boston and Harvard Medical School, Boston, MA 02115, USA.
Antje-Christin DeppeDepartment of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, Germany.
Maximilian SchernerDepartment of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, Germany.
Thorsten WittwerDepartment of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, Germany ; Center for Molecular Medicine Cologne, University of Cologne, 50931 Cologne, Germany.
Jürgen HeschelerInstitute for Neurophysiology, University of Cologne, 50931 Cologne, Germany.
Thorsten WahlersDepartment of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, Germany ; Center for Molecular Medicine Cologne, University of Cologne, 50931 Cologne, Germany.
Yeong-Hoon ChoiDepartment of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, Germany ; Center for Molecular Medicine Cologne, University of Cologne, 50931 Cologne, Germany.
University of Cologne · DEBerlin-Brandenburger Centrum für Regenerative Therapien · DEHarvard University · US

Funding

Engineering of Pacemaker Tissue for Cardiac ImplantationR01HL068915 · NHLBI · CHILDREN'S HOSPITAL BOSTON · PI COWAN, DOUGLAS BURR · 2002 to 2009
$2.9M
Expansion and Directed Differentiation of Adult Side Population Stem CellsR01HL088206 · NHLBI · BOSTON CHILDREN'S HOSPITAL · PI COWAN, DOUGLAS BURR · 2007 to 2011
$1.9M
NHLBI NIH HHS R01 HL068915NHLBI NIH HHS R01 HL088206
6 · The paper itself

Abstract

Ischemic heart disease is the main cause of death in western countries and its burden is increasing worldwide. It typically involves irreversible degeneration and loss of myocardial tissue leading to poor prognosis and fatal outcome. Autologous cells with the potential to regenerate damaged heart tissue would be an ideal source for cell therapeutic approaches. Here, we compared different methods of conditional culture for increasing the yield and cardiogenic potential of murine skeletal muscle-derived stem cells. A subpopulation of nonadherent cells was isolated from skeletal muscle by preplating and applying cell culture conditions differing in support of cluster formation. In contrast to static culture conditions, dynamic culture with or without previous hanging drop preculture led to significantly increased cluster diameters and the expression of cardiac specific markers on the protein and mRNA level. Whole-cell patch-clamp studies revealed similarities to pacemaker action potentials and responsiveness to cardiac specific pharmacological stimuli. This data indicates that skeletal muscle-derived stem cells are capable of adopting enhanced cardiac muscle cell-like properties by applying specific culture conditions. Choosing this route for the establishment of a sustainable, autologous source of cells for cardiac therapies holds the potential of being clinically more acceptable than transgenic manipulation of cells.

Identifiers

PMID26357517
PMCPMC4556334
OpenAlexW2119827711

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.