ArticleNature protocols2015
Whole-mount immunolocalization to study female meiosis in Arabidopsis.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- The plant early recombinosome: a high security complex to break DNA during meiosis.Plant reproduction · 2024Review
- Signaling by the EPFL-ERECTA family coordinates female germline specification through the BZR1 family in Arabidopsis.The Plant cell · 2023Article
- Spatiotemporal formation of the large vacuole regulated by the BIN2-VLG module is required for female gametophyte development in Arabidopsis.The Plant cell · 2023Article
- Crossover interference mechanism: New lessons from plants.Frontiers in cell and developmental biology · 2023Review
- Specification of female germline by microRNA orchestrated auxin signaling in Arabidopsis.Nature communications · 2022Article
- Splicing-mediated activation of SHAGGY-like kinases underpinning carbon partitioning in Arabidopsis seeds.The Plant cell · 2022Article
- The Inactivation of ArabidopsisPlants (Basel, Switzerland) · 2021Article
- Characterization of germline development and identification of genes associated with germline specification in pineapple.Horticulture research · 2021Article
- High-throughput single-cell transcriptomics reveals the female germline differentiation trajectory in Arabidopsis thaliana.Communications biology · 2021Article
- The Formation of Bivalents and the Control of Plant Meiotic Recombination.Frontiers in plant science · 2021Review
- From Microscopy to Nanoscopy: Defining anFrontiers in plant science · 2021Review
- Regulation of Female Germline Specification via Small RNA Mobility in Arabidopsis.The Plant cell · 2020Article
- Simple Whole-Mount Staining Protocol of F-Actin for Studies of the Female Gametophyte in Agavoideae and Other Crassinucellate Ovules.Frontiers in plant science · 2020Article
- MeioCapture: an efficient method for staging and isolation of meiocytes in the prophase I sub-stages of meiosis in wheat.BMC plant biology · 2018Article
- Arabidopsis ICK/KRP cyclin-dependent kinase inhibitors function to ensure the formation of one megaspore mother cell and one functional megaspore per ovule.PLoS genetics · 2018Article
- Article
- The THO Complex Non-Cell-Autonomously Represses Female Germline Specification through the TAS3-ARF3 Module.Current biology : CB · 2017Article
- Amplifying recombination genome-wide and reshaping crossover landscapes in Brassicas.PLoS genetics · 2017Article
- A Multigenic Network of ARGONAUTE4 Clade Members Controls Early Megaspore Formation in Arabidopsis.Genetics · 2016Article
- New observations on gametogenic development and reproductive experimental tools to support seed yield improvement in cowpea [Vigna unguiculata (L.) Walp].Plant reproduction · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Here we describe a whole-mount immunolocalization protocol to follow the subcellular localization of proteins during female meiosis in Arabidopsis thaliana, a model species that is used to study sexual reproduction in flowering plants. By using confocal microscopy, the procedure allows one to follow megasporogenesis at all stages before differentiation of the functional megaspore. This in particular includes stages that occur during prophase I, such as the installation of the axial and central elements of the synaptonemal complex along the meiotic chromosomes. In contrast to procedures that require microtome sectioning or enzymatic isolation and smearing to separate female meiocytes from neighboring cells, this 3-day protocol preserves the constitution of the developing primordium and incorporates the architecture of the ovule to provide a temporal and spatial context to meiotic divisions. This opens up the possibility to systematically compare the dynamics of protein localization during female and male meiosis. Steps describe tissue collection and fixation, preparation of slides and polyacrylamide embedding, tissue permeabilization, antibody incubation, propidium iodide staining, and finally image acquisition by confocal microscopy. The procedure adds an essential technique to the toolkit of plant meiotic analysis, and it represents a framework for technical adaptations that could soon allow the analysis of plant reproductive alternatives to sexual reproduction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.