ArticleNature protocols2015
Studying tumor growth in Drosophila using the tissue allograft method.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 50 citations in OpenAlex.
- A tumor-suppressive role of the PRC1 Polycomb epigenetic complex in the maintenance of adult Drosophila intestinal stem cell identity.PLoS genetics · 2026Article
- Tumor-Associated Macrophages Promote Brain Metastasis.bioRxiv : the preprint server for biology · 2026Article
- FromProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Beyond Mice and Men: Alternative Vertebrate and Invertebrate Models in Cancer Biology.Annual review of cancer biology · 2026Article
- Beyond the mouse: 3R-guided alternative animal models transforming cancer research.Molecular cancer · 2026Review
- Allograft transplantation for Drosophila tumor metastasis studies.Disease models & mechanisms · 2025Article
- 3D nanoprinting of embryo microinjection needles with anti-clogging features.Microsystems & nanoengineering · 2025Article
- Expanding the CRISPR/Cas toolkit: applications in proteomics and theranostics.Frontiers in bioengineering and biotechnology · 2025Review
- Drosophila as a Model for Metastasis.Advances in experimental medicine and biology · 2025Review
- The Power of Drosophila in Modeling Cancer Cachexia.Advances in experimental medicine and biology · 2025Review
- TrxT and dhd are dispensable for Drosophila brain development but essential for l(3)mbt brain tumour growth.EMBO reports · 2024Article
- Article
- Growth deregulation and interaction with host hemocytes contribute to tumor progression in a Drosophila brain tumor model.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Drosophila innate immunity suppresses the survival of xenografted mammalian tumor cells.Scientific reports · 2023Article
- Modelling Cancer Metastasis inCells · 2023Review
- Fruit fly for anticancer drug discovery and repurposing.Annals of medicine and surgery (2012) · 2023Article
- Illuminati: a form of gene expression plasticity in Drosophila neural stem cells.Development (Cambridge, England) · 2022Article
- Tumour-host interactions through the lens of Drosophila.Nature reviews. Cancer · 2021Review
- Tumor-induced disruption of the blood-brain barrier promotes host death.Developmental cell · 2021Article
- Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This protocol describes a method to allograft Drosophila larval tissue into adult fly hosts that can be used to assay the tumorigenic potential of mutant tissues. The tissue of interest is dissected, loaded into a fine glass needle and implanted into a host. Upon implantation, nontransformed tissues do not overgrow beyond their normal size, but malignant tumors grow without limit, are invasive and kill the host. By using this method, Drosophila malignant tumors can be transplanted repeatedly, for years, and therefore they can be aged beyond the short life span of flies. Because several hosts can be implanted using different pieces from a single tumor, the method also allows the tumor mass to be increased to facilitate further studies that may require large amounts of tissue (i.e., genomics, proteomics and so on). This method also provides an operational definition of hyperplastic, benign and malignant growth. The injection procedure itself requires only ∼1 d. Tumor development can then be monitored until the death of the implanted hosts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.