ArticlePloS one2015
Measuring Meiotic Crossovers via Multi-Locus Genotyping of Single Pollen Grains in Barley.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- A simple and high-throughput single pollen genotyping pipeline to study meiosis in apple (Malus x domestica).Plant methods · 2026Article
- Crystal Digital PCR™-Based Single Nucleus Pollen Genotyping in Barley (Hordeum vulgare L.) to Measure Meiotic Crossover Rates in Defined Chromosomal Intervals.Methods in molecular biology (Clifton, N.J.) · 2026Article
- The Control of the Crossover Localization in Allium.International journal of molecular sciences · 2023Article
- Genomic prediction of the recombination rate variation in barley - A route to highly recombinogenic genotypes.Plant biotechnology journal · 2022Article
- Measuring the frequency and distribution of meiotic crossovers in homozygous barley inbred lines.Frontiers in plant science · 2022Article
- Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering.Nature communications · 2020Article
- Analysis of Crossover Events and Allele Segregation Distortion in Interspecific Citrus Hybrids by Single Pollen Genotyping.Frontiers in plant science · 2020Article
- More Filtering on SNP Calling Does Not Remove Evidence of Inter-Nucleus Recombination in Dikaryotic Arbuscular Mycorrhizal Fungi.Frontiers in plant science · 2020Article
- Variation in Recombination Rate Is Shaped by Domestication and Environmental Conditions in Barley.Molecular biology and evolution · 2019Article
- Together But Different: The Subgenomes of the BimodalFrontiers in plant science · 2019Article
- Assessing Ploidy Level Analysis and Single Pollen Genotyping of Diploid and Euploid Citrus Genotypes by Fluorescence-Activated Cell Sorting and Whole-Genome Amplification.Frontiers in plant science · 2019Article
- The genetic architecture of genome-wide recombination rate variation in allopolyploid wheat revealed by nested association mapping.The Plant journal : for cell and molecular biology · 2018Article
- Tackling Plant Meiosis: From Model Research to Crop Improvement.Frontiers in plant science · 2018Review
- Sequencing of Single Pollen Nuclei Reveals Meiotic Recombination Events at Megabase Resolution and Circumvents Segregation Distortion Caused by Postmeiotic Processes.Frontiers in plant science · 2017Article
- Evidence for the sexual origin of heterokaryosis in arbuscular mycorrhizal fungi.Nature microbiology · 2016Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The detection of meiotic crossovers in crop plants currently relies on scoring DNA markers in a segregating population or cytological visualization. We investigated the feasibility of using flow-sorted haploid nuclei, Phi29 DNA polymerase-based whole-genome-amplification (WGA) and multi-locus KASP-genotyping to measure meiotic crossovers in individual barley pollen grains. To demonstrate the proof of concept, we used 24 gene-based physically mapped single nucleotide polymorphisms to genotype the WGA products of 50 single pollen nuclei. The number of crossovers per chromosome, recombination frequencies along chromosome 3H and segregation distortion were analysed and compared to a doubled haploid (DH) population of the same genotype. The number of crossovers and chromosome wide recombination frequencies show that this approach is able to produce results that resemble those obtained from other methods in a biologically meaningful way. Only the segregation distortion was found to be lower in the pollen population than in DH plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.