ArticleScientific reports2015
High-throughput sequencing and morphology perform equally well for benthic monitoring of marine ecosystems.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
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Who cites it
49 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-analysis shows both congruence and complementarity of DNA and eDNA metabarcoding to traditional methods for biological community assessment.Molecular ecology · 2022Pooled it
- Formulated Diet Instead of Chilled Fish Is Beneficial to Microbial and Water Quality in Mandarin Fish (Siniperca chuatsi) Ponds.Current microbiology · 2026Article
- Metabarcoding and targeted barcoding can enhance Norwegian Continental Shelf macrofauna species inventories.PeerJ · 2026Article
- Different brewing materials change the vertical distribution pattern of eukaryotic communities in Luzhou-flavor liquor pit muds.Scientific reports · 2025Article
- Review
- Evaluation of Oxford Nanopore Technologies MinION Sequencer as a Novel Short Amplicon Metabarcoding Tool Using Arthropod Mock Sample and Irish Bat Diet Characterisation.Ecology and evolution · 2025Article
- The Core of the Matter-Importance of Identification Method and Biological Replication for Benthic Marine Monitoring.Ecology and evolution · 2024Article
- Biodiversity of microorganisms in the Baltic Sea: the power of novel methods in the identification of marine microbes.FEMS microbiology reviews · 2024Review
- Assessing the Seasonal and Spatial Dynamics of Zooplankton through DNA Metabarcoding in a Temperate Estuary.Animals : an open access journal from MDPI · 2023Article
- Community dynamics of microbial eukaryotes in intertidal mudflats in the hypertidal Bay of Fundy.ISME communications · 2023Article
- Patterns of eukaryotic diversity from the surface to the deep-ocean sediment.Science advances · 2022Article
- Fine-scale differences in eukaryotic communities inside and outside salmon aquaculture cages revealed by eDNA metabarcoding.Frontiers in genetics · 2022Article
- Diversity and composition of macroinvertebrate communities in a rare inland salt marsh.Ecology and evolution · 2021Article
- Ecosystems monitoring powered by environmental genomics: A review of current strategies with an implementation roadmap.Molecular ecology · 2021Review
- Monitoring the ecological status of rivers with diatom eDNA metabarcoding: A comparison of taxonomic markers and analytical approaches for the inference of a molecular diatom index.Molecular ecology · 2021Article
- Article
- Biofilm and planktonic microbial communities in highly acidic soil (pH < 3) in the Soos National Nature Reserve, Czech Republic.Extremophiles : life under extreme conditions · 2020Article
- Nourished, Exposed Beaches Exhibit Altered Sediment Structure and Meiofaunal Communities.Diversity · 2020Article
- Population-level inferences from environmental DNA-Current status and future perspectives.Evolutionary applications · 2020Review
- Spatiotemporal Patterns in Diversity and Assembly Process of Marine Protist Communities of the Changjiang (Yangtze River) Plume and Its Adjacent Waters.Frontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Environmental diversity surveys are crucial for the bioassessment of anthropogenic impacts on marine ecosystems. Traditional benthic monitoring relying on morphotaxonomic inventories of macrofaunal communities is expensive, time-consuming and expertise-demanding. High-throughput sequencing of environmental DNA barcodes (metabarcoding) offers an alternative to describe biological communities. However, whether the metabarcoding approach meets the quality standards of benthic monitoring remains to be tested. Here, we compared morphological and eDNA/RNA-based inventories of metazoans from samples collected at 10 stations around a fish farm in Scotland, including near-cage and distant zones. For each of 5 replicate samples per station, we sequenced the V4 region of the 18S rRNA gene using the Illumina technology. After filtering, we obtained 841,766 metazoan sequences clustered in 163 Operational Taxonomic Units (OTUs). We assigned the OTUs by combining local BLAST searches with phylogenetic analyses. We calculated two commonly used indices: the Infaunal Trophic Index and the AZTI Marine Biotic Index. We found that the molecular data faithfully reflect the morphology-based indices and provides an equivalent assessment of the impact associated with fish farms activities. We advocate that future benthic monitoring should integrate metabarcoding as a rapid and accurate tool for the evaluation of the quality of marine benthic ecosystems.
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