Evidence map›Paper›PMID 26355095›Full record

ArticleJournal of virology2015

The Nucleoprotein Is Required for Lymphocytic Choriomeningitis Virus-Based Vaccine Vector Immunogenicity.

Stephanie Darbre, Susan Johnson, Sandra Kallert, Paul-Henri Lambert, Claire-Anne Siegrist, Daniel D Pinschewer

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
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  3. Review
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  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Stephanie DarbreDepartment of Pathology and Immunology, University of Geneva, Geneva, Switzerland World Health Organization Collaborating Centre for Vaccine Immunology, University of Geneva, Geneva, Switzerland.
Susan JohnsonDepartment of Pathology and Immunology, University of Geneva, Geneva, Switzerland World Health Organization Collaborating Centre for Vaccine Immunology, University of Geneva, Geneva, Switzerland.
Sandra KallertDepartment of Pathology and Immunology, University of Geneva, Geneva, Switzerland World Health Organization Collaborating Centre for Vaccine Immunology, University of Geneva, Geneva, Switzerland Division of Experimental Virology, Department of Biomedicine, University of Basel, Basel, Switzerland.
Paul-Henri LambertDepartment of Pathology and Immunology, University of Geneva, Geneva, Switzerland World Health Organization Collaborating Centre for Vaccine Immunology, University of Geneva, Geneva, Switzerland.
Claire-Anne SiegristDepartment of Pathology and Immunology, University of Geneva, Geneva, Switzerland World Health Organization Collaborating Centre for Vaccine Immunology, University of Geneva, Geneva, Switzerland.
Daniel D PinschewerDepartment of Pathology and Immunology, University of Geneva, Geneva, Switzerland World Health Organization Collaborating Centre for Vaccine Immunology, University of Geneva, Geneva, Switzerland Division of Experimental Virology, Department of Biomedicine, University of Basel, Basel, Switzerland daniel.pinschewer@gmx.ch.
University of Geneva · CHWorld Health Organization · CH

Funding

European Research Council 310962
6 · The paper itself

Abstract

Recombinant glycoprotein-deficient lymphocytic choriomeningitis virus-based vaccine vectors (rLCMV/ΔGP) are potent CD8(+) T cell inducers. To investigate the underlying molecular requirements, we generated a nucleoprotein-deficient vector counterpart (rLCMV/ΔNP). NP but not GP is a minimal trans-acting factor for viral transcription and genome replication. We found that, unlike rLCMV/ΔGP, rLCMV/ΔNP failed to elicit detectable CD8(+) T cell responses unless NP was trans complemented in a transgenic host. Hence, NP-dependent intracellular gene expression is essential for LCMV vector immunogenicity.

Indexed as

3' Untranslated RegionsAnimalsCD8-Positive T-LymphocytesCell LineCricetinaeGene ExpressionLymphocytic ChoriomeningitisLymphocytic choriomeningitis virusMiceMice, TransgenicNucleoproteinsViral Vaccines3' Untranslated RegionsNucleoproteinsViral Vaccines

Identifiers

PMID26355095
PMCPMC4645649
OpenAlexW1874164675

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.