ArticleThe Journal of biological chemistry2015
Rapid deamination of cyclobutane pyrimidine dimer photoproducts at TCG sites in a translationally and rotationally positioned nucleosome in vivo.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Genome-wide maps of CPD deamination in yeast reveal the impact of DNA sequence context and nucleosome architecture on cytosine deamination rates.Genome research · 2026Article
- UVA-induced DNA damage and mutations in human melanocytes: relevance for melanoma mutations.Nucleic acids research · 2025Article
- Mutation rate heterogeneity at the sub-gene scale due to local DNA hypomethylation.Nucleic acids research · 2024Article
- High UV damage and low repair, but not cytosine deamination, stimulate mutation hotspots at ETS binding sites in melanoma.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Post- and Pre-Radiolabeling Assays forBiochemistry · 2023Article
- Acceleration of the Deamination of Cytosine through Photo-Crosslinking.Current issues in molecular biology · 2023Article
- Participation of Histones in DNA Damage and Repair within Nucleosome Core Particles: Mechanism and Applications.Accounts of chemical research · 2022Article
- Article
- Damage mapping techniques and the light they have shed on canonical and atypical UV photoproducts.Frontiers in genetics · 2022Review
- The major mechanism of melanoma mutations is based on deamination of cytosine in pyrimidine dimers as determined by circle damage sequencing.Science advances · 2021Article
- The epigenetic DNA modification 5-carboxylcytosine promotes high levels of cyclobutane pyrimidine dimer formation upon UVB irradiation.Genome instability & disease · 2021Article
- Mechanisms of UV-induced mutations and skin cancer.Genome instability & disease · 2020Article
- Biochemical reconstitution of UV-induced mutational processes.Nucleic acids research · 2019Article
- Oxidation of 8-Oxo-7,8-dihydro-2'-deoxyguanosine Leads to Substantial DNA-Histone Cross-Links within Nucleosome Core Particles.Chemical research in toxicology · 2018Article
- Nucleosome positions establish an extended mutation signature in melanoma.PLoS genetics · 2018Article
- Enhanced Cleavage at Abasic Sites within Clustered Lesions in Nucleosome Core Particles.Chembiochem : a European journal of chemical biology · 2018Article
- Article
- Hydroxyl-radical footprinting combined with molecular modeling identifies unique features of DNA conformation and nucleosome positioning.Nucleic acids research · 2017Article
- The interaction between cytosine methylation and processes of DNA replication and repair shape the mutational landscape of cancer genomes.Nucleic acids research · 2017Article
- Differential DNA repair underlies mutation hotspots at active promoters in cancer genomes.Nature · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Sunlight-induced C to T mutation hot spots in skin cancers occur primarily at methylated CpG sites that coincide with sites of UV-induced cyclobutane pyrimidine dimer (CPD) formation. The C and 5-methyl-C in CPDs are not stable and deaminate to U and T, respectively, which leads to the insertion of A by the DNA damage bypass polymerase η, thereby defining a probable mechanism for the origin of UV-induced C to T mutations. Deamination rates for T(m)CG CPDs have been found to vary 12-fold with rotational position in a nucleosome in vitro. To determine the influence of nucleosome structure on deamination rates in vivo, we determined the deamination rates of CPDs at TCG sites in a stably positioned nucleosome within the FOS promoter in HeLa cells. A procedure for in vivo hydroxyl radical footprinting with Fe-EDTA was developed, and, together with results from a cytosine methylation protection assay, we determined the translational and rotational positions of the TCG sites. Consistent with the in vitro observations, deamination was slower for one CPD located at an intermediate rotational position compared with two other sites located at outside positions, and all were much faster than for CPDs at non-TCG sites. Photoproduct formation was also highly suppressed at one site, possibly due to its interaction with a histone tail. Thus, it was shown that CPDs of TCG sites deaminate the fastest in vivo and that nucleosomes can modulate both their formation and deamination, which could contribute to the UV mutation hot spots and cold spots.
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