ArticleAnnals of biomedical engineering2016
Bioimaging: An Useful Tool to Monitor Differentiation of Human Embryonic Stem Cells into Chondrocytes.
Article in Annals of biomedical engineering, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Dickkopf-3 enhances the chondroprotective effect of chondrogenic progenitor cells in a rat model of posttraumatic osteoarthritis.Journal of orthopaedic surgery and research · 2025Article
- ColMA-based bioprinted 3D scaffold allowed to study tenogenic events in human tendon stem cells.Bioengineering & translational medicine · 2025Article
- 3DFrontiers in bioengineering and biotechnology · 2022Article
- Chondrogenic Commitment of Human Bone Marrow Mesenchymal Stem Cells in a Perfused Collagen Hydrogel Functionalized with hTGF-β1-Releasing PLGA Microcarrier.Pharmaceutics · 2021Article
- Chondrogenic Differentiation of Pluripotent Stem Cells under Controllable Serum-Free Conditions.International journal of molecular sciences · 2019Article
- Integrated Multi-Assay Culture Model for Stem Cell Chondrogenic Differentiation.International journal of molecular sciences · 2019Article
- Effect of cellular mass on chondrogenic differentiation during embryoid body formation.Molecular medicine reports · 2018Article
- The Good the Bad and the Ugly of Glycosaminoglycans in Tissue Engineering Applications.Pharmaceuticals (Basel, Switzerland) · 2017Review
- Gene expression profile in human induced pluripotent stem cells: Chondrogenic differentiation in vitro, part A.Molecular medicine reports · 2017Article
- Gene expression profile in human induced pluripotent stem cells: Chondrogenic differentiation in vitro, part B.Molecular medicine reports · 2017Article
- Comparison of the early response of human embryonic stem cells and human induced pluripotent stem cells to ionizing radiation.Molecular medicine reports · 2017Article
- Comparison of Four Protocols to Generate Chondrocyte-Like Cells from Human Induced Pluripotent Stem Cells (hiPSCs).Stem cell reviews and reports · 2017Article
- Therapeutic potential of mesenchymal stem cell based therapy for osteoarthritis.Clinical and translational medicine · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To improve the recovery of damaged cartilage tissue, pluripotent stem cell-based therapies are being intensively explored. A number of techniques exist that enable monitoring of stem cell differentiation, including immunofluorescence staining. This simple and fast method enables changes to be observed during the differentiation process. Here, two protocols for the differentiation of human embryonic stem cells into chondrocytes were used (monolayer cell culture and embryoid body formation). Cells were labeled for markers expressed during the differentiation process at different time points (pluripotent: NANOG, SOX2, OCT3/4, E-cadherin; prochondrogenic: SOX6, SOX9, Collagen type II; extracellular matrix components: chondroitin sulfate, heparan sulfate; beta-catenin, CXCR4, and Brachyury). Comparison of the signal intensity of differentiated cells to control cell populations (articular cartilage chondrocytes and human embryonic stem cells) showed decreased signal intensities of pluripotent markers, E-cadherin and beta-catenin. Increased signal intensities of prochondrogenic markers and extracellular matrix components were observed. The changes during chondrogenic differentiation monitored by evaluation of pluripotent and chondrogenic markers signal intensity were described. The changes were similar to several studies over chondrogenesis. These results were confirmed by semi-quantitative analysis of IF signals. In this research we indicate a bioimaging as a useful tool to monitor and semi-quantify the IF pictures during the differentiation of hES into chondrocyte-like.
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