ArticleNature communications2015
Outbred genome sequencing and CRISPR/Cas9 gene editing in butterflies.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
72 citing papers in PubMed.
- Combining Annotation Software to Identify Orthologous Genes (CASIO) Provides a New Dataset of Orthologous Genes for Swallowtail Butterflies.Molecular ecology resources · 2026Article
- Mechanisms of Aristolochic Acid Resistance in Specialist Butterflies and Evolutionary Insights for Potential Protective Pathways.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Whole genome analysis of toxic Papilionidae butterflies utilizing aristolochic acid, Pachliopta aristolochiae, and Byasa alcinous.DNA research : an international journal for rapid publication of reports on genes and genomes · 2026Article
- High-Humidity Incubation Improves Post-Microinjection Viability and Larval Performance of CRISPR/Cas9-EditedInsects · 2025Article
- Chromosome level genome assembly of tea tussock moth, Euproctis pseudoconspersa.Scientific data · 2025Article
- Highly Efficient Generation of Germline Mutations Using CRISPR/Cas9 in the Speckled Wood ButterflyEcology and evolution · 2025Article
- Article
- Establishment of CRISPR/Cas9-based knock-in in a hemimetabolous insect: targeted gene tagging in the cricket Gryllus bimaculatus.Development (Cambridge, England) · 2025Article
- Manipulation of a New Non-model Insect Genome Using Targeted CRISPR-Era Approaches.Methods in molecular biology (Clifton, N.J.) · 2025Article
- TheGenes · 2024Article
- Egg Cooling After Oviposition Extends the Permissive Period for Microinjection-Mediated Genome Modification inInternational journal of molecular sciences · 2024Article
- Evolutionary patterns and functional effects of 3D chromatin structures in butterflies with extensive genome rearrangements.Nature communications · 2024Article
- ContScout: sensitive detection and removal of contamination from annotated genomes.Nature communications · 2024Article
- Dispersal from the Qinghai-Tibet plateau by a high-altitude butterfly is associated with rapid expansion and reorganization of its genome.Nature communications · 2023Article
- Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control inInsects · 2023Article
- Genomics of the relict species Baronia brevicornis sheds light on its demographic history and genome size evolution across swallowtail butterflies.G3 (Bethesda, Md.) · 2023Article
- Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management.Plants (Basel, Switzerland) · 2023Review
- Chromosome-Level Genome Assembly ofInsects · 2023Article
- Mutagenesis of the odorant receptor co-receptor (Orco) reveals severe olfactory defects in the crop pest moth Helicoverpa armigera.BMC biology · 2022Article
- Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
24 authors.
Funding
Abstract
Butterflies are exceptionally diverse but their potential as an experimental system has been limited by the difficulty of deciphering heterozygous genomes and a lack of genetic manipulation technology. Here we use a hybrid assembly approach to construct high-quality reference genomes for Papilio xuthus (contig and scaffold N50: 492 kb, 3.4 Mb) and Papilio machaon (contig and scaffold N50: 81 kb, 1.15 Mb), highly heterozygous species that differ in host plant affiliations, and adult and larval colour patterns. Integrating comparative genomics and analyses of gene expression yields multiple insights into butterfly evolution, including potential roles of specific genes in recent diversification. To functionally test gene function, we develop an efficient (up to 92.5%) CRISPR/Cas9 gene editing method that yields obvious phenotypes with three genes, Abdominal-B, ebony and frizzled. Our results provide valuable genomic and technological resources for butterflies and unlock their potential as a genetic model system.
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Registered trials
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