ArticlePlant physiology2015
Reticulomics: Protein-Protein Interaction Studies with Two Plasmodesmata-Localized Reticulon Family Proteins Identify Binding Partners Enriched at Plasmodesmata, Endoplasmic Reticulum, and the Plasma Membrane.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 95 citations in OpenAlex.
- An ER-microtubule bridge: Reticulon 17 links microtubules with ER network organisation in plants.The Plant journal : for cell and molecular biology · 2026Article
- The study of plasmodesmal biology using proximity labeling technologies.Journal of experimental botany · 2026Review
- FRET-FLIM: Unveiling Transcription Factor Interactions in Plants.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Concerted transport and phosphorylation of diacylglycerol at ER-PM contact sites regulate phospholipid dynamics during stress.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The Extended Synaptotagmins ofPlants (Basel, Switzerland) · 2025Review
- Tobacco Mosaic Virus Movement: From Capsid Disassembly to Transport Through Plasmodesmata.Viruses · 2025Review
- Cellular Partners of Tobamoviral Movement Proteins.International journal of molecular sciences · 2025Review
- FRET-FLIM for the Study of Protein-Protein Interactions Underpinning Mitosis Checkpoints.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Two plant membrane-shaping reticulon-like proteins play contrasting complex roles in turnip mosaic virus infection.Molecular plant pathology · 2024Article
- Advances in understanding the graft healing mechanism: a review of factors and regulatory pathways.Horticulture research · 2024Article
- Nonspecific phospholipases C3 and C4 interact with PIN-FORMED2 to regulate growth and tropic responses in Arabidopsis.The Plant cell · 2024Article
- Seasonal dynamics of cell-to-cell transport in angiosperm wood.Journal of experimental botany · 2024Article
- Communicating Across Cell Walls: Structure, Evolution, and Regulation of Plasmodesmatal Transport in Plants.Results and problems in cell differentiation · 2024Review
- Immunoprecipitation and FRET-FLIM to Determine Metabolons on the Plant ER.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Make It Shine: Labelling the ER for Light and Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- In Vivo Analysis of ER-Associated Protein Degradation and Ubiquitination in Arabidopsis thaliana.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Recombinant expression and subcellular targeting of the particulate methane monooxygenase (pMMO) protein components in plants.Scientific reports · 2023Article
- Exo84c interacts with VAP27 to regulate exocytotic compartment degradation and stigma senescence.Nature communications · 2023Article
- Comparative transcriptional and co-expression network analysis of two upland cotton accessions with extreme phenotypic differences reveals molecular mechanisms of fiber development.Frontiers in plant science · 2023Article
- Reticulons 3 and 6 interact with viral movement proteins.Molecular plant pathology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The endoplasmic reticulum (ER) is a ubiquitous organelle that plays roles in secretory protein production, folding, quality control, and lipid biosynthesis. The cortical ER in plants is pleomorphic and structured as a tubular network capable of morphing into flat cisternae, mainly at three-way junctions, and back to tubules. Plant reticulon family proteins (RTNLB) tubulate the ER by dimerization and oligomerization, creating localized ER membrane tensions that result in membrane curvature. Some RTNLB ER-shaping proteins are present in the plasmodesmata (PD) proteome and may contribute to the formation of the desmotubule, the axial ER-derived structure that traverses primary PD. Here, we investigate the binding partners of two PD-resident reticulon proteins, RTNLB3 and RTNLB6, that are located in primary PD at cytokinesis in tobacco (Nicotiana tabacum). Coimmunoprecipitation of green fluorescent protein-tagged RTNLB3 and RTNLB6 followed by mass spectrometry detected a high percentage of known PD-localized proteins as well as plasma membrane proteins with putative membrane-anchoring roles. Förster resonance energy transfer by fluorescence lifetime imaging microscopy assays revealed a highly significant interaction of the detected PD proteins with the bait RTNLB proteins. Our data suggest that RTNLB proteins, in addition to a role in ER modeling, may play important roles in linking the cortical ER to the plasma membrane.
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