ArticlePLoS genetics2015
Outer Hair Cell Lateral Wall Structure Constrains the Mobility of Plasma Membrane Proteins.
Article in PLoS genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Verification of Outer Hair Cell Motor Protein, Prestin, as a Serological Biomarker for Mouse Cochlear Damage.International journal of molecular sciences · 2024Article
- Cochlear transcriptome analysis of an outbred mouse population (CFW).Frontiers in cellular neuroscience · 2023Article
- Prestin-Mediated Frequency Selectivity Does not Cover Ultrahigh Frequencies in Mice.Neuroscience bulletin · 2022Article
- Protocol to assess two distinct components of the nonlinear capacitance in mouse cardiomyocytes.STAR protocols · 2021Article
- Prestin amplifies cardiac motor functions.Cell reports · 2021Article
- Expression of a membrane-targeted fluorescent reporter disrupts auditory hair cell mechanoelectrical transduction and causes profound deafness.Hearing research · 2021Article
- Maturation of Voltage-induced Shifts in SLC26a5 (Prestin) Operating Point during Trafficking and Membrane Insertion.Neuroscience · 2020Article
- Recent advances in cochlear hair cell nanophysiology: subcellular compartmentalization of electrical signaling in compact sensory cells.Faculty reviews · 2020Review
- The awesome power of optogenetics in hearing research.Neuroscience letters · 2019Review
- Deletion of exons 17 and 18 in prestin's STAS domain results in loss of function.Scientific reports · 2019Article
- Modeling of the axon plasma membrane structure and its effects on protein diffusion.PLoS computational biology · 2019Article
- High-resolution transcriptional dissection of in vivo Atoh1-mediated hair cell conversion in mature cochleae identifies Isl1 as a co-reprogramming factor.PLoS genetics · 2018Article
- Prestin Contributes to Membrane Compartmentalization and Is Required for Normal Innervation of Outer Hair Cells.Frontiers in cellular neuroscience · 2018Article
- Complex polymorphisms in endocytosis genes suggest alpha-cyclodextrin as a treatment for breast cancer.PloS one · 2018Article
- Cyclodextrins and Iatrogenic Hearing Loss: New Drugs with Significant Risk.Frontiers in cellular neuroscience · 2017Review
- Article
- Membrane prestin expression correlates with the magnitude of prestin-associated charge movement.Hearing research · 2016Article
- Susceptibility of outer hair cells to cholesterol chelator 2-hydroxypropyl-β-cyclodextrine is prestin-dependent.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
19 authors at 6 institutions in 3 countries.
Funding
Abstract
Nature's fastest motors are the cochlear outer hair cells (OHCs). These sensory cells use a membrane protein, Slc26a5 (prestin), to generate mechanical force at high frequencies, which is essential for explaining the exquisite hearing sensitivity of mammalian ears. Previous studies suggest that Slc26a5 continuously diffuses within the membrane, but how can a freely moving motor protein effectively convey forces critical for hearing? To provide direct evidence in OHCs for freely moving Slc26a5 molecules, we created a knockin mouse where Slc26a5 is fused with YFP. These mice and four other strains expressing fluorescently labeled membrane proteins were used to examine their lateral diffusion in the OHC lateral wall. All five proteins showed minimal diffusion, but did move after pharmacological disruption of membrane-associated structures with a cholesterol-depleting agent and salicylate. Thus, our results demonstrate that OHC lateral wall structure constrains the mobility of plasma membrane proteins and that the integrity of such membrane-associated structures are critical for Slc26a5's active and structural roles. The structural constraint of membrane proteins may exemplify convergent evolution of cellular motors across species. Our findings also suggest a possible mechanism for disorders of cholesterol metabolism with hearing loss such as Niemann-Pick Type C diseases.
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Registered trials
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