ArticleAnalytical chemistry2015
Reducing adsorption to improve recovery and in vivo detection of neuropeptides by microdialysis with LC-MS.
Article in Analytical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 53 citations in OpenAlex.
- Minimally Invasive Chemical Biopsy Needle with Self-Wettable Extraction Phase For In Vivo Tissue Sampling During Medical Procedures.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Neuropeptides in the Extracellular Space of the Mouse Cortex Measured In Vivo by Nanodialysis Probe Coupled with LC-MS.Angewandte Chemie (International ed. in English) · 2025Article
- Electrochemical Quantification of Enkephalin Peptides Using Fast-Scan Cyclic Voltammetry.Analytical chemistry · 2024Article
- Current Challenges and Future Directions in Peptidomics.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Recent advances in mass spectrometry analysis of neuropeptides.Mass spectrometry reviews · 2023Review
- Brain pharmacokinetics of mono- and bispecific amyloid-β antibodies in wild-type and Alzheimer's disease mice measured by high cut-off microdialysis.Fluids and barriers of the CNS · 2022Article
- Emerging approaches for decoding neuropeptide transmission.Trends in neurosciences · 2022Review
- Reduction of αSYN Pathology in a Mouse Model of PD Using a Brain-Penetrating Bispecific Antibody.Pharmaceutics · 2022Article
- Multiplexed Monitoring of Neurochemicals via Electrografting-Enabled Site-Selective Functionalization of Aptamers on Field-Effect Transistors.Analytical chemistry · 2022Article
- Letting the little light of mind shine: Advances and future directions in neurochemical detection.Neuroscience research · 2022Review
- Wireless, battery-free push-pull microsystem for membrane-free neurochemical sampling in freely moving animals.Science advances · 2022Article
- LC-MS/MS Analysis Elucidates a Daily Rhythm in Orexin A Concentration in the Rat Vitreous Body.Molecules (Basel, Switzerland) · 2021Article
- Mass Spectrometry Quantification, Localization, and Discovery of Feeding-Related Neuropeptides inACS chemical neuroscience · 2021Article
- Sampling insulin in different tissue compartments using microdialysis: methodological aspects.Scientific reports · 2020Article
- Dynorphin and its role in alcohol use disorder.Brain research · 2020Review
- Blood-Based Biomarkers of Quinpirole Pharmacology: Cluster-Based PK/PD and Metabolomics to Unravel the Underlying Dynamics in Rat Plasma and Brain.CPT: pharmacometrics & systems pharmacology · 2019Article
- Article
- On-Column Dimethylation with Capillary Liquid Chromatography-Tandem Mass Spectrometry for Online Determination of Neuropeptides in Rat Brain Microdialysate.Analytical chemistry · 2018Article
- Microdialysis Coupled with LC-MS/MS for In Vivo Neurochemical Monitoring.The AAPS journal · 2017Article
- Analytical Techniques in Neuroscience: Recent Advances in Imaging, Separation, and Electrochemical Methods.Analytical chemistry · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Neuropeptides are an important class of neurochemicals; however, measuring their concentration in vivo by using microdialysis sampling is challenging due to their low concentration and the small samples generated. Capillary liquid chromatography with mass spectrometry (cLC-MS) can yield attomole limits of detection (LOD); however, low recovery and loss of sample to adsorptive surfaces can still hinder detection of neuropeptides. We have evaluated recovery during sampling and transfer to the cLC column for a selection of 10 neuropeptides. Adding acetonitrile to sample eliminated carryover and improved LOD by 1.4- to 60-fold. The amount of acetonitrile required was found to have an optimal value that correlated with peptide molecular weight and retention time on a reversed phase LC column. Treating AN69 dialysis membrane, which bears negative charge due to incorporated sulfonate groups, with polyethylenimine (PEI) improved recovery by 1.2- to 80-fold. The effect appeared to be due to reducing electrostatic interaction between peptides and the microdialysis probe because modification increased recovery only for peptides that carried net positive charge. The combined effects improved LOD of the entire method by 1.3- to 800-fold for the different peptides. We conclude that peptides with both charged and hydrophobic regions require combined strategies to prevent adsorption and yield the best possible detection. The method was demonstrated by determining orexin A, orexin B, and a novel isoform of rat β-endorphin in the arcuate nucleus. Dialysate concentrations were below 10 pM for these peptides. A standard addition study on dialysates revealed that while some peptides can be accurately quantified, some are affected by the matrix.
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Registered trials
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