ArticleProtoplasma2016
Engineering overexpression of ORCA3 and strictosidine glucosidase in Catharanthus roseus hairy roots increases alkaloid production.
Article in Protoplasma, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 61 citations in OpenAlex.
- Co-Overexpression ofPlants (Basel, Switzerland) · 2026Article
- Explant origin and growth regulators as determinants of callus performance and terpenoid indole alkaloid accumulation in Catharanthus roseus.BMC plant biology · 2026Article
- A chromosome-level Mitragyna parvifolia genome unveils spirooxindole alkaloid diversification and mitraphylline biosynthesis.The Plant cell · 2025Article
- Visual analysis of research progress and trend on hairy roots.Frontiers in plant science · 2025Article
- Strategies, Achievements, and Potential Challenges of Plant and Microbial Chassis in the Biosynthesis of Plant Secondary Metabolites.Molecules (Basel, Switzerland) · 2024Review
- TheAoB PLANTS · 2024Article
- Ethyl Methane Sulfonate and Sodium Azide-Mediated Chemical and X-ray-Mediated Physical Mutagenesis Positively RegulatePlants (Basel, Switzerland) · 2022Article
- Interplay of transcription factors orchestrating the biosynthesis of plant alkaloids.3 Biotech · 2022Review
- Application of metabolic engineering to enhance the content of alkaloids in medicinal plants.Metabolic engineering communications · 2022Review
- Comparative transcriptome analysis revealed the molecular mechanism of the effect of light intensity on the accumulation of rhynchophylline and isorhynchophylline inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Genetic Manipulation and Bioreactor Culture of Plants as a Tool for Industry and Its Applications.Molecules (Basel, Switzerland) · 2022Review
- Hairy roots: An untapped potential for production of plant products.Frontiers in plant science · 2022Review
- Transcription Factors in Alkaloid Engineering.Biomolecules · 2021Review
- Production of bioactive plant secondary metabolites through in vitro technologies-status and outlook.Applied microbiology and biotechnology · 2021Review
- A source-sink model explains the difference in the metabolic mechanism of mechanical damage to young and senescing leaves in Catharanthus roseus.BMC plant biology · 2021Article
- Plant Secondary Metabolite Transporters: Diversity, Functionality, and Their Modulation.Frontiers in plant science · 2021Review
- Plant tissue culture as a perpetual source for production of industrially important bioactive compounds.Biotechnology reports (Amsterdam, Netherlands) · 2020Review
- Hairy Root Cultures-A Versatile Tool With Multiple Applications.Frontiers in plant science · 2020Review
- CrERF5, an AP2/ERF Transcription Factor, Positively Regulates the Biosynthesis of Bisindole Alkaloids and Their Precursors inFrontiers in plant science · 2019Article
- A Novel AP2/ERF Transcription Factor CR1 Regulates the Accumulation of Vindoline and Serpentine inFrontiers in plant science · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Catharanthus roseus produces many pharmaceutically important terpenoid indole alkaloids (TIAs) such as vinblastine, vincristine, ajmalicine, and serpentine. Past metabolic engineering efforts have pointed to the tight regulation of the TIA pathway and to multiple rate-limiting reactions. Transcriptional regulator ORCA3 (octadecanoid responsive Catharanthus AP2-domain protein), activated by jasmonic acid, plays a central role in regulating the TIA pathway. In this study, overexpressing ORCA3 under the control of a glucocorticoid-inducible promoter in C. roseus hairy roots resulted in no change in the total amount of TIAs measured. RT-qPCR results showed that ORCA3 overexpression triggered the upregulation of transcripts of most of the known TIA pathway genes. One notable exception was the decrease in strictosidine glucosidase (SGD) transcripts. These results corresponded to previously published results. In this study, ORCA3 and SGD were both engineered in hairy roots under the control of a glucocorticoid-inducible promoter. Co-overexpression of ORCA3 and SGD resulted in a significant (p < 0.05) increase in serpentine by 44 %, ajmalicine by 32 %, catharanthine by 38 %, tabersonine by 40 %, lochnericine by 60 % and hörhammericine by 56 % . The total alkaloid pool was increased significantly by 47 %. Thus, combining overexpression of a positive regulator and a pathway gene which is not controlled by this regulator provided a way to enhance alkaloid production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.