Evidence map›Paper›PMID 26350435›Full record

ArticleCellular microbiology2016

Antibacterial effect of mesenchymal stem cells against Escherichia coli is mediated by secretion of beta- defensin- 2 via toll- like receptor 4 signalling.

Dong Kyung Sung, Yun Sil Chang, Se In Sung, Hye Soo Yoo, So Yoon Ahn, Won Soon Park

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
104citing papers in PubMed, 1 pooled it
6.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

104 citing papers in PubMed, 1 synthesis or guideline pooled it, 163 citations in OpenAlex.

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44 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Dong Kyung SungSamsung Biomedical Research Institute, Seoul, 136- 701, Korea.
Yun Sil ChangDepartment of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, 135- 710, Korea.
Se In SungDepartment of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, 135- 710, Korea.
Hye Soo YooDepartment of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, 135- 710, Korea.
So Yoon AhnDepartment of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, 135- 710, Korea.
Won Soon ParkDepartment of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, 135- 710, Korea.
Samsung (South Korea) · KRSamsung Medical Center · KRSungkyunkwan University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recently, we demonstrated that intratracheal transplantation of human umbilical cord blood- derived mesenchymal stem cells (MSCs) attenuates Escherichia (E) coli- induced acute lung injury primarily by down- modulating inflammation and enhancing bacterial clearance iQn mice. This study was performed to elucidate the mechanism underlying the antibacterial effects of MSCs. The growth of E. coli in vitro was significantly inhibited only by MSCs or their conditioned medium with bacterial preconditioning, but not by fibroblasts or their conditioned medium. Microarray analysis identified significant up- regulation of toll- like receptors (TLR)- 2 and TLR- 4, and β- defensin 2 (BD2) in MSCs compared with fibroblasts after E. coli exposure. The increased BD2 level and the in vitro antibacterial effects of MSCs were abolished by specific antagonist or by siRNA- mediated knockdown of TLR- 4, but not TLR- 2, and restored by BD2 supplementation. The in vivo down- modulation of the inflammatory response and enhanced bacterial clearance, increased BD2 secretion and the resultant protection against E. coli- induced pneumonia observed only with MSCs, but not fibroblasts, transplantation in mice, were abolished by knockdown of TLR- 4 with siRNA transfection. Our data indicate that BD2 secreted by the MSCs via the TLR- 4 signalling pathway is one of the critical paracrine factors mediating their microbicidal effects against E. coli, both in vitro and in vivo. Furthermore, TLR- 4 from the transplanted MSCs plays a seminal role in attenuating in vivo E. coli- induced pneumonia and the ensuing acute lung injury through both its anti- inflammatory and antibacterial effects.

Indexed as

Host-Pathogen InteractionsAcute Lung InjuryAnimalsbeta-DefensinsEscherichia coliEscherichia coli InfectionsFemaleGene Expression RegulationHumansMaleMesenchymal Stem CellsMesenchymal Stem Cell TransplantationMice, Inbred ICRPneumonia, BacterialPregnancyToll-Like Receptor 4beta-DefensinsDEFB4A protein, humanTLR4 protein, humanToll-Like Receptor 4acute lung injurybeta- defensin 2Escherichia colimesenchymal stem cellstoll- like receptor 4

Identifiers

PMID26350435
PMCPMC5057339
OpenAlexW2117220410

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.