ArticleJournal of cell science2015
Analytical cell adhesion chromatography reveals impaired persistence of metastatic cell rolling adhesion to P-selectin.
Article in Journal of cell science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Single-cell adhesive profiling in an optofluidic device elucidates CD8Cell reports methods · 2024Article
- Microengineered In Vitro Assays for Screening and Sorting Manufactured Therapeutic T Cells.Journal of immunology (Baltimore, Md. : 1950) · 2024Review
- Cholesterol depletion decreases adhesion of non-small cell lung cancer cells to E-selectin.American journal of physiology. Cell physiology · 2023Article
- Adhesion analysis via a tumor vasculature-like microfluidic device identifies CD8Cell reports · 2023Article
- Quantitative interpretation of cell rolling velocity distribution.Biophysical journal · 2021Article
- Lymph Node Subcapsular Sinus Microenvironment-On-A-Chip Modeling Shear Flow Relevant to Lymphatic Metastasis and Immune Cell Homing.iScience · 2020Article
- Modular microfluidic systems cast from 3D-printed molds for imaging leukocyte adherence to differentially treated endothelial cultures.Scientific reports · 2019Article
- Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues.Scientific reports · 2019Article
- Analyzing Mechanisms of Metastatic Cancer Cell Adhesive Phenotype Leveraging Preparative Adhesion Chromatography Microfluidic.Advanced biosystems · 2019Article
- Fluorometric Quantification of Single-Cell Velocities to Investigate Cancer Metastasis.Cell systems · 2018Article
- P-, but not E- or L-, selectin-mediated rolling adhesion persistence in hemodynamic flow diverges between metastatic and leukocytic cells.Oncotarget · 2017Article
- P-Selectin and ICAM-1 synergy in mediating THP-1 monocyte adhesion in hemodynamic flow is length dependent.Integrative biology : quantitative biosciences from nano to macro · 2017Article
- Implications of Lymphatic Transport to Lymph Nodes in Immunity and Immunotherapy.Annual review of biomedical engineering · 2016Review
- Force and torque on spherical particles in micro-channel flows using computational fluid dynamics.Royal Society open science · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Selectins facilitate the recruitment of circulating cells from the bloodstream by mediating rolling adhesion, which initiates the cell-cell signaling that directs extravasation into surrounding tissues. To measure the relative efficiency of cell adhesion in shear flow for in vitro drug screening, we designed and implemented a microfluidic-based analytical cell adhesion chromatography system. The juxtaposition of instantaneous rolling velocities with elution times revealed that human metastatic cancer cells, but not human leukocytes, had a reduced capacity to sustain rolling adhesion with P-selectin. We define a new parameter, termed adhesion persistence, which is conceptually similar to migration persistence in the context of chemotaxis, but instead describes the capacity of cells to resist the influence of shear flow and sustain rolling interactions with an adhesive substrate that might modulate the probability of extravasation. Among cell types assayed, adhesion persistence to P-selectin was specifically reduced in metastatic but not leukocyte-like cells in response to a low dose of heparin. In conclusion, we demonstrate this as an effective methodology to identify selectin adhesion antagonist doses that modulate homing cell adhesion and engraftment in a cell-subtype-selective manner.
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Registered trials
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