ArticleScientific reports2015
The identification of an integral membrane, cytochrome c urate oxidase completes the catalytic repertoire of a therapeutic enzyme.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 24 citations in OpenAlex.
- Status, challenges, and prospects of artificial intelligence application in gout diagnosis and treatment, drug research and development, and disease monitoring.Frontiers in medicine · 2026Review
- A reductive uric acid degradation pathway in anaerobic bacteria.Life metabolism · 2025Article
- In Hyphomicrobium denitrificans Two Related Sulfane-Sulfur Responsive Transcriptional Repressors Regulate Thiosulfate Oxidation and Have a Deep Impact on Nitrate Respiration and Anaerobic Biosyntheses.Molecular microbiology · 2025Article
- Systematic Engineering for Efficient Uric Acid-Degrading Activity in Probiotic YeastACS synthetic biology · 2025Article
- Paradigms of convergent evolution in enzymes.The FEBS journal · 2025Article
- Multiphase Radical Chemical Processes Induced by Air Pollutants and the Associated Health Effects.Environment & health (Washington, D.C.) · 2025Review
- A Functional Analysis of the Purine Salvage Pathway in Acetobacter fabarum.Journal of bacteriology · 2022Article
- Identification of a Formate-Dependent Uric Acid Degradation Pathway inJournal of bacteriology · 2019Article
- Herbivorous turtle ants obtain essential nutrients from a conserved nitrogen-recycling gut microbiome.Nature communications · 2018Article
- Catalysis and Structure of Zebrafish Urate Oxidase Provide Insights into the Origin of Hyperuricemia in Hominoids.Scientific reports · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In living organisms, the conversion of urate into allantoin requires three consecutive enzymes. The pathway was lost in hominid, predisposing humans to hyperuricemia and gout. Among other species, the genomic distribution of the two last enzymes of the pathway is wider than that of urate oxidase (Uox), suggesting the presence of unknown genes encoding Uox. Here we combine gene network analysis with association rule learning to identify the missing urate oxidase. In contrast with the known soluble Uox, the identified gene (puuD) encodes a membrane protein with a C-terminal cytochrome c. The 8-helix transmembrane domain corresponds to DUF989, a family without similarity to known proteins. Gene deletion in a PuuD-encoding organism (Agrobacterium fabrum) abolished urate degradation capacity; the phenotype was fully restored by complementation with a cytosolic Uox from zebrafish. Consistent with H2O2 production by zfUox, urate oxidation in the complemented strain caused a four-fold increase of catalase. No increase was observed in the wild-type, suggesting that urate oxidation by PuuD proceeds through cytochrome c-mediated electron transfer. These findings identify a missing link in purine catabolism, assign a biochemical activity to a domain of unknown function (DUF989), and complete the catalytic repertoire of an enzyme useful for human therapy.
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