ArticleInvestigative ophthalmology & visual science2015
In Vivo Imaging of Retinal Oxidative Stress Using a Reactive Oxygen Species-Activated Fluorescent Probe.
Article in Investigative ophthalmology & visual science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Delivery of Lipid Nanoparticles with ROS Probes for Improved Visualization of Hepatocellular Carcinoma.Biomedicines · 2023Article
- Polyunsaturated Lipids in the Light-Exposed and Prooxidant Retinal Environment.Antioxidants (Basel, Switzerland) · 2023Review
- MicroRNA Expression Analysis of Mice Retinas with Oxygen-Induced Retinopathy by RNA Sequencing.Journal of ophthalmology · 2022Article
- Photoreceptor cells and RPE contribute to the development of diabetic retinopathy.Progress in retinal and eye research · 2021Review
- Retinal oxidative stress activates the NRF2/ARE pathway: An early endogenous protective response to ocular hypertension.Redox biology · 2021Article
- Elemental iron modifies the redox environment of the gastrointestinal tract: A novel therapeutic target and test for metabolic syndrome.Free radical biology & medicine · 2021Article
- MicroRNA-301a-3p promotes diabetic retinopathy via regulation of six-transmembrane epithelial antigen of prostate 4.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2021Article
- Detection of Vascular Reactive Oxygen Species in Experimental Atherosclerosis by High-Resolution Near-Infrared Fluorescence Imaging Using VCAM-1-Targeted Liposomes Entrapping a Fluorogenic Redox-Sensitive Probe.Oxidative medicine and cellular longevity · 2021Article
- Non-invasive real-time imaging of reactive oxygen species (ROS) using auto-fluorescence multispectral imaging technique: A novel tool for redox biology.Redox biology · 2020Article
- Photodynamic Therapy Is an Effective Adjuvant Therapy for Image-Guided Surgery in Prostate Cancer.Cancer research · 2020Article
- Imaging Retinal Activity in the Living Eye.Annual review of vision science · 2019Review
- Challenges and Opportunities for Small-Molecule Fluorescent Probes in Redox Biology Applications.Antioxidants & redox signaling · 2018Review
- Oxidative stress measured in vivo without an exogenous contrast agent using QUEST MRI.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2018Review
- Formation of 53BP1 foci and ATM activation under oxidative stress is facilitated by RNA:DNA hybrids and loss of ATM-53BP1 expression promotes photoreceptor cell survival in mice.F1000Research · 2018Article
- Grape polyphenols reduce gut-localized reactive oxygen species associated with the development of metabolic syndrome in mice.PloS one · 2018Article
- Roles of miRNAs and long noncoding RNAs in the progression of diabetic retinopathy.Bioscience reports · 2017Review
- Elevated oxidative stress in the aortic media of patients with bicuspid aortic valve.The Journal of thoracic and cardiovascular surgery · 2017Article
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Rapid repeatable in vivo detection of retinal reactive oxygen species.Experimental eye research · 2017Article
- Enhancing the efficacy of AREDS antioxidants in light-induced retinal degeneration.Molecular vision · 2017Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
purposeIn vivo methods for detecting oxidative stress in the eye would improve screening and monitoring of the leading causes of blindness: diabetic retinopathy, glaucoma, and age-related macular degeneration.
methodsTo develop an in vivo biomarker for oxidative stress in the eye, we tested the efficacy of a reactive oxygen species (ROS)-activated, near-infrared hydrocyanine-800CW (H-800CW) fluorescent probe in light-induced retinal degeneration (LIRD) mouse models. After intravitreal delivery in LIRD rats, fluorescent microscopy was used to confirm that the oxidized H-800CW appeared in the same retinal layers as an established ROS marker (dichlorofluorescein).
resultsDose-response curves of increasing concentrations of intravenously injected H-800CW demonstrated linear increases in both intensity and total area of fundus hyperfluorescence in LIRD mice, as detected by scanning laser ophthalmoscopy. Fundus hyperfluorescence also correlated with the duration of light damage and functional deficits in vision after LIRD. In LIRD rats with intravitreal injections of H-800CW, fluorescent labeling was localized to photoreceptor inner segments, similar to dichlorofluorescein.
conclusionsHydrocyanine-800CW detects retinal ROS in vivo and shows potential as a novel biomarker for ROS levels in ophthalmic diseases.
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