ArticleScientific reports2015
Activation of bacterial channel MscL in mechanically stimulated droplet interface bilayers.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 46 citations in OpenAlex.
- The Ion Permeability of DNA Nanotube Channels.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Challenges and opportunities in achieving the full potential of droplet interface bilayers.Nature chemistry · 2022Review
- Enhancing membrane-based soft materials with magnetic reconfiguration events.Scientific reports · 2022Article
- Negative Influence by the Force: Mechanically Induced Hyperpolarization via KInternational journal of molecular sciences · 2021Review
- Manufacture of Multilayered Artificial Cell Membranes through Sequential Bilayer Deposition on Emulsion Templates.Chembiochem : a European journal of chemical biology · 2021Article
- Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology.Membranes · 2021Review
- Surface energy and separation mechanics of droplet interface phospholipid bilayers.Journal of the Royal Society, Interface · 2021Article
- Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry.Chemical science · 2021Article
- Membrane protein mediated bilayer communication in networks of droplet interface bilayers.Communications chemistry · 2020Article
- Engineering the Osmotic State ofFrontiers in bioengineering and biotechnology · 2020Article
- The voltage-dependence of MscL has dipolar and dielectric contributions and is governed by local intramembrane electric field.Scientific reports · 2018Article
- Reconfiguring droplet interface bilayer networks through sacrificial membranes.Biomicrofluidics · 2018Article
- Encapsulating Networks of Droplet Interface Bilayers in a Thermoreversible Organogel.Scientific reports · 2018Article
- Membrane Perfusion of Hydrophobic Substances Around Channels Embedded in the Contact Bubble Bilayer.Scientific reports · 2017Article
- Activation of the mechanosensitive ion channel MscL by mechanical stimulation of supported Droplet-Hydrogel bilayers.Scientific reports · 2017Article
- Piezo1 Channels Are Inherently Mechanosensitive.Cell reports · 2016Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MscL, a stretch-activated channel, saves bacteria experiencing hypo-osmotic shocks from lysis. Its high conductance and controllable activation makes it a strong candidate to serve as a transducer in stimuli-responsive biomolecular materials. Droplet interface bilayers (DIBs), flexible insulating scaffolds for such materials, can be used as a new platform for incorporation and activation of MscL. Here, we report the first reconstitution and activation of the low-threshold V23T mutant of MscL in a DIB as a response to axial compressions of the droplets. Gating occurs near maximum compression of both droplets where tension in the membrane is maximal. The observed 0.1-3 nS conductance levels correspond to the V23T-MscL sub-conductive and fully open states recorded in native bacterial membranes or liposomes. Geometrical analysis of droplets during compression indicates that both contact angle and total area of the water-oil interfaces contribute to the generation of tension in the bilayer. The measured expansion of the interfaces by 2.5% is predicted to generate a 4-6 mN/m tension in the bilayer, just sufficient for gating. This work clarifies the principles of interconversion between bulk and surface forces in the DIB, facilitates the measurements of fundamental membrane properties, and improves our understanding of MscL response to membrane tension.
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