ArticlePloS one2015
A Circadian Clock Gene, Cry, Affects Heart Morphogenesis and Function in Drosophila as Revealed by Optical Coherence Microscopy.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Attention LSTM U-Net model forBiomedical optics express · 2024Article
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- Open-source, highly efficient, post-acquisition synchronization for 4D dual-contrast imaging of the mouse embryonic heart over development with optical coherence tomography.Biomedical optics express · 2023Article
- Roles of peripheral clocks: lessons from the fly.FEBS letters · 2022Review
- Effects of an electric field on sleep quality and life span mediated by ultraviolet (UV)-A/blue light photoreceptor CRYPTOCHROME in Drosophila.Scientific reports · 2021Article
- ELAC2/RNaseZ-linked cardiac hypertrophy in Drosophila melanogaster.Disease models & mechanisms · 2021Article
- Live mechanistic assessment of localized cardiac pumping in mammalian tubular embryonic heart.Journal of biomedical optics · 2020Article
- Non-invasive red-light optogenetic control of Drosophila cardiac function.Communications biology · 2020Article
- Label-free optical imaging in developmental biology [Invited].Biomedical optics express · 2020Review
- Segmentation of Drosophila heart in optical coherence microscopy images using convolutional neural networks.Journal of biophotonics · 2018Article
- Speckle variance optical coherence tomography of blood flow in the beating mouse embryonic heart.Journal of biophotonics · 2017Article
- Article
- Blue-light induced accumulation of reactive oxygen species is a consequence of the Drosophila cryptochrome photocycle.PloS one · 2017Article
- Dynamic imaging and quantitative analysis of cranial neural tube closure in the mouse embryo using optical coherence tomography.Biomedical optics express · 2017Article
- Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM).Journal of visualized experiments : JoVE · 2016Article
- Circadian clock and the onset of cardiovascular events.Hypertension research : official journal of the Japanese Society of Hypertension · 2016Review
- Optogenetic pacing in Drosophila melanogaster.Science advances · 2015Article
- Optical Coherence Tomography for Brain Imaging and Developmental Biology.IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics SocietyArticle
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Circadian rhythms are endogenous, entrainable oscillations of physical, mental and behavioural processes in response to local environmental cues such as daylight, which are present in the living beings, including humans. Circadian rhythms have been related to cardiovascular function and pathology. However, the role that circadian clock genes play in heart development and function in a whole animal in vivo are poorly understood. The Drosophila cryptochrome (dCry) is a circadian clock gene that encodes a major component of the circadian clock negative feedback loop. Compared to the embryonic stage, the relative expression levels of dCry showed a significant increase (>100-fold) in Drosophila during the pupa and adult stages. In this study, we utilized an ultrahigh resolution optical coherence microscopy (OCM) system to perform non-invasive and longitudinal analysis of functional and morphological changes in the Drosophila heart throughout its post-embryonic lifecycle for the first time. The Drosophila heart exhibited major morphological and functional alterations during its development. Notably, heart rate (HR) and cardiac activity period (CAP) of Drosophila showed significant variations during the pupa stage, when heart remodeling took place. From the M-mode (2D + time) OCM images, cardiac structural and functional parameters of Drosophila at different developmental stages were quantitatively determined. In order to study the functional role of dCry on Drosophila heart development, we silenced dCry by RNAi in the Drosophila heart and mesoderm, and quantitatively measured heart morphology and function in those flies throughout its development. Silencing of dCry resulted in slower HR, reduced CAP, smaller heart chamber size, pupal lethality and disrupted posterior segmentation that was related to increased expression of a posterior compartment protein, wingless. Collectively, our studies provided novel evidence that the circadian clock gene, dCry, plays an essential role in heart morphogenesis and function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.