Evidence map›Paper›PMID 26347880›Full record

ArticleBioMed research international2015

A Locked Nucleic Acid Probe Based on Selective Salt-Induced Effect Detects Single Nucleotide Polymorphisms.

Jing Zhang, Huizhe Wu, Qiuchen Chen, Pengfei Zhao, Haishan Zhao, Weifan Yao, Minjie Wei

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 97% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Jing ZhangDepartment of Pharmacology, School of Pharmacy, China Medical University, Liaoning Key Laboratory of Molecular Targeted Anti-Tumor Drug Development and Evaluation, No. 77 Puhe Road, Shenbei District, Shenyang 110013, China.
Huizhe WuDepartment of Pharmacology, School of Pharmacy, China Medical University, Liaoning Key Laboratory of Molecular Targeted Anti-Tumor Drug Development and Evaluation, No. 77 Puhe Road, Shenbei District, Shenyang 110013, China.
Qiuchen ChenDepartment of Pharmacology, School of Pharmacy, China Medical University, Liaoning Key Laboratory of Molecular Targeted Anti-Tumor Drug Development and Evaluation, No. 77 Puhe Road, Shenbei District, Shenyang 110013, China.
Pengfei ZhaoDepartment of Pharmacology, School of Pharmacy, China Medical University, Liaoning Key Laboratory of Molecular Targeted Anti-Tumor Drug Development and Evaluation, No. 77 Puhe Road, Shenbei District, Shenyang 110013, China.
Haishan ZhaoDepartment of Pharmacology, School of Pharmacy, China Medical University, Liaoning Key Laboratory of Molecular Targeted Anti-Tumor Drug Development and Evaluation, No. 77 Puhe Road, Shenbei District, Shenyang 110013, China.
Weifan YaoDepartment of Pharmacology, School of Pharmacy, China Medical University, Liaoning Key Laboratory of Molecular Targeted Anti-Tumor Drug Development and Evaluation, No. 77 Puhe Road, Shenbei District, Shenyang 110013, China.
Minjie WeiDepartment of Pharmacology, School of Pharmacy, China Medical University, Liaoning Key Laboratory of Molecular Targeted Anti-Tumor Drug Development and Evaluation, No. 77 Puhe Road, Shenbei District, Shenyang 110013, China.
China Medical University · CN

Funding

HSRD VA RCS 14-232
6 · The paper itself

Abstract

Detection of single based genetic mutation by using oligonucleotide probes is one of the common methods of detecting single nucleotide polymorphisms at known loci. In this paper, we demonstrated a hybridization system which included a buffer solution that produced selective salt-induced effect and a locked nucleic acid modified 12 nt oligonucleotide probe. The hybridization system is suitable for hybridization under room temperature. By using magnetic nanoparticles as carriers for PCR products, the SNPs (MDR1 C3435T/A) from 45 volunteers were analyzed, and the results were consistent with the results from pyrophosphoric acid sequencing. The method presented in this paper differs from the traditional method of using molecular beacons to detect SNPs in that it is suitable for research institutions lacking real-time quantitative PCR detecting systems, to detect PCR products at room temperature.

Indexed as

Polymorphism, Single NucleotideATP Binding Cassette Transporter, Subfamily BDNA ProbesFemaleHumansMaleNucleic Acid HybridizationPolymerase Chain ReactionABCB1 protein, humanATP Binding Cassette Transporter, Subfamily BDNA Probes

Identifiers

PMID26347880
PMCPMC4548076
OpenAlexW1720182698

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.