ArticleThe Journal of experimental biology2015
Hyperpolarized 13C NMR observation of lactate kinetics in skeletal muscle.
Article in The Journal of experimental biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 45 citations in OpenAlex.
- Differential activation of TCA cycle activity and PDH flux by dichloroacetate in skeletal muscle measured by hyperpolarized [2-iScience · 2026Article
- Hyperpolarized NMR study of the impact of alzheimer's disease on diabetes using a novel rat model.Journal of physiology and biochemistry · 2026Article
- HyperpolarizedNMR in biomedicine · 2025Article
- The mitochondrial lactate oxidation complex: endpoint for carbohydrate carbon disposal.American journal of physiology. Endocrinology and metabolism · 2025Article
- Operational stability study of lactate biosensors: modeling, parameter identification, and stability analysis.Frontiers in bioengineering and biotechnology · 2024Article
- Tracing the lactate shuttle to the mitochondrial reticulum.Experimental & molecular medicine · 2022Review
- Lactate in contemporary biology: a phoenix risen.The Journal of physiology · 2022Article
- DynamicMagnetic resonance in medicine · 2022Article
- Labile Photo-Induced Free Radical in α-Ketoglutaric Acid: a Universal Endogenous Polarizing Agent for In Vivo HyperpolarizedAngewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2022Article
- Labile Photo-Induced Free Radical in α-Ketoglutaric Acid: a Universal Endogenous Polarizing Agent for In Vivo HyperpolarizedAngewandte Chemie (International ed. in English) · 2022Article
- The blood lactate/pyruvate equilibrium affair.American journal of physiology. Endocrinology and metabolism · 2022Article
- Simple Esterification of [1-ACS chemical biology · 2021Article
- Hyperpolarized NMR study of the impact of pyruvate dehydrogenase kinase inhibition on the pyruvate dehydrogenase and TCA flux in type 2 diabetic rat muscle.Pflugers Archiv : European journal of physiology · 2021Article
- HyperpolarizedRadiology · 2021Article
- The "Anaerobic Threshold" Concept Is Not Valid in Physiology and Medicine.Medicine and science in sports and exercise · 2021Article
- Comprehensive Literature Review of Hyperpolarized Carbon-13 MRI: The Road to Clinical Application.Metabolites · 2021Review
- Assessment of hepatic pyruvate carboxylase activity using hyperpolarized [1-Magnetic resonance in medicine · 2021Article
- High fieldPhysical chemistry chemical physics : PCCP · 2021Article
- HyperpolarizedBiomedicines · 2021Article
- Role of the Heart in Lactate Shuttling.Frontiers in nutrition · 2021Review
Corrections and comments
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Authors and funding
8 authors at 6 institutions in 3 countries.
Funding
Abstract
The production of glycolytic end products, such as lactate, usually evokes a cellular shift from aerobic to anaerobic ATP generation and O2 insufficiency. In the classical view, muscle lactate must be exported to the liver for clearance. However, lactate also forms under well-oxygenated conditions, and this has led investigators to postulate lactate shuttling from non-oxidative to oxidative muscle fiber, where it can serve as a precursor. Indeed, the intracellular lactate shuttle and the glycogen shunt hypotheses expand the vision to include a dynamic mobilization and utilization of lactate during a muscle contraction cycle. Testing the tenability of these provocative ideas during a rapid contraction cycle has posed a technical challenge. The present study reports the use of hyperpolarized [1-(13)C]lactate and [2-(13)C]pyruvate in dynamic nuclear polarization (DNP) NMR experiments to measure the rapid pyruvate and lactate kinetics in rat muscle. With a 3 s temporal resolution, (13)C DNP NMR detects both [1-(13)C]lactate and [2-(13)C]pyruvate kinetics in muscle. Infusion of dichloroacetate stimulates pyruvate dehydrogenase activity and shifts the kinetics toward oxidative metabolism. Bicarbonate formation from [1-(13)C]lactate increases sharply and acetyl-l-carnitine, acetoacetate and glutamate levels also rise. Such a quick mobilization of pyruvate and lactate toward oxidative metabolism supports the postulated role of lactate in the glycogen shunt and the intracellular lactate shuttle models. The study thus introduces an innovative DNP approach to measure metabolite transients, which will help delineate the cellular and physiological role of lactate and glycolytic end products.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.